Proteome analysis, renaturation and reutilization of carious dentin

龋齿牙本质的蛋白质组分析、复性和再利用

• 批准号: 18592084
• 负责人: SHIMADA Yasushi
• 金额: $ 2.39万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18592084/
• 关键词: carious dentin; proteome analysis; composite resin; renaturation; reutilization; ナノリーケージ; セルフエッチングプライマー; レーザー

First, we investigated the bonding capacity of current resin adhesives to caries-affected dentin. The bond strength showed lower values than that of the normal dentin, which were significantly influenced by the caries removal method.Then we evaluated the nanoleakage formation along the resin-dentin adhesive interface in order to determine the sealing ability of the adhesives. The penetration of silver particle as a tracer indicated the location of nanoleakage, even at the interface with normal dentin. Different nanoleakage expressions were revealed among the adhesives used, as well as the location of dentin. Especially, deep cavity preparation revealed distinct nanoleakage.We also investigated the localization of matrix metalloprotainases (MMPs) in carious dentin including bacterial infected zone and non-infected zone, as well as in sound dentin using immunoelectron microscopy. Frozen sections of human dentin with caries were labeled by IgG conjugated with gold colloidal particles, and quantitative analysis was performed. The level of MMP-2 showed no significant difference among the three regions, while MMP-8 and MMP-9 decreased at inner caries, but intensified again at outer caries. MMP-8 and MMP-9 from saliva may participate in matrix breakdown of outer caries. Additionally, we immersed the resin adhesive specimens bonded to artificially demineralized tooth surfaces in phosphate buffered fluoride solution and evaluated the effect of fluoride uptake on the resin bonding. The bond strength of resin specimens was significantly increased after the immersion in the fluoride solution. We prepared dual-paste calcium phosphate cement (CPC) that forms hydroxyapatite (HA) as a final product, in order to improve the handling in clinic. Although the mechanical property of the cement was not so strong, we anticipated the CPC to be HA forming material for caries treatment.

首先，我们研究了当前树脂粘合剂对受龋齿影响的牙本质的粘合能力。粘合强度显示出低于正常牙本质的值，这受到除龋方法的显着影响。然后我们评估了沿着树脂-牙本质粘合剂界面的纳米渗漏形成，以确定粘合剂的密封能力。银颗粒作为示踪剂的渗透表明了纳米渗漏的位置，甚至在与正常牙本质的界面处也是如此。所使用的粘合剂以及牙本质的位置揭示了不同的纳米渗漏表现。特别是深腔制备显示出明显的纳米渗漏。我们还使用免疫电子显微镜研究了龋齿牙本质（包括细菌感染区和非感染区）以及健全牙本质中基质金属蛋白酶（MMP）的定位。将人龋齿牙本质冰冻切片用胶体金颗粒缀合的IgG进行标记，并进行定量分析。 MMP-2的水平在三个区域之间没有显着差异，而MMP-8和MMP-9在内龋处下降，但在外龋处再次增强。唾液中的 MMP-8 和 MMP-9 可能参与外龋齿的基质分解。此外，我们将与人工脱矿牙齿表面粘合的树脂粘合剂样本浸入磷酸盐缓冲氟化物溶液中，并评估氟化物吸收对树脂粘合的影响。在氟化物溶液中浸泡后，树脂试样的结合强度显着增加。我们制备了双糊状磷酸钙骨水泥（CPC），最终产品形成羟基磷灰石（HA），以改善临床操作。虽然水门汀的机械性能不是那么强，但我们预计CPC可以成为治疗龋齿的HA形成材料。

项目成果

The localization of matrix metalloprotainases in sound and cariesdentin

基质金属蛋白酶在健全牙本质和龋牙本质中的定位

• 发表时间: 2007
• 作者: Shimada Y;Ichinose S;Yuan Y;Sadr A;Tagami J
• 通讯作者: Tagami J

Effect of dentin depth and bonding tactics on hybridization quality

牙本质深度和粘接策略对杂交质量的影响

• 发表时间: 2007
• 作者: Yuan Y;Shimada Y;Ichinose S;Sasafuchi Y;Tagami J
• 通讯作者: Tagami J

The localization of matrix metalloprotainases in sound and caries dentin

基质金属蛋白酶在健全牙本质和龋牙本质中的定位

• 发表时间: 2007
• 作者: Shimada Y;Ichinose S;Yuan Y;Sadr A;Tagami J
• 通讯作者: Tagami J

Effect of fluoride in phosphate buffer solution on bonding to artificially carious enamel

磷酸盐缓冲溶液中的氟化物对人工龋牙釉质粘结的影响

• 发表时间: 2007
• 期刊: Dental Materials Journal 26
• 作者: Wang H;Shimada Y;Tagami J
• 通讯作者: Tagami J

Sear bond strength of tooth-colored indirect restorations bonded to mid-coronal and cervical dentin

牙色间接修复体与中冠和颈部牙本质粘合的灼烧粘合强度

• 发表时间: 2006
• 期刊: Dental Materials Journal 25
• 作者: Shimada Y;Sattabanasuk V;Sadr A;Yuan Y;He Z;Tagami J
• 通讯作者: Tagami J