Evaluation of liver damage by direct pulp capping and development of functional materials for pulp capping with biocompatibility and tissue degeneration

光交联明胶-磷酸普鲁兰多糖直接盖髓法在牙科领域得到了广泛应用。在大鼠第一磨牙制备具有暴露牙髓区域的洞。使用1/4圆棒形成空腔，并侵蚀纸浆。用10%次氯酸钠和3%过氧化氢冲洗制备的腔体。将所有洞分为三组。暴露的纸浆被光交联明胶制剂、磷普鲁兰制剂覆盖，并被常规的氢氧化钙制剂覆盖。然后用光照射覆盖有可光交联的明胶制剂的腔。磨牙治疗区域的边缘用保护衬垫密封。分别于术后1周和2周处死动物。术后1周、2周观察三组戴帽牙的情况。在洞制备后1周，在氢氧化钙制剂、光交联明胶制剂和磷酸普鲁兰多糖制剂处理的牙齿中，在牙髓暴露部位下观察到炎性细胞的聚结。我们发现光交联明胶制剂和磷酸普鲁兰多糖制剂治疗龋洞时炎症反应较轻。洞制备后2周，氢氧化钙制剂和磷酸普鲁兰多糖制剂促进钙化。探讨光固化明胶和磷普鲁兰用于直接盖髓的效果。

The photo-crosslinkable gelatin and phosphopullulan was employed as direct pulp capping method in dental field. Cavities with the exposed pulp area were prepared of first molars in rats. The cavity was formed using a 1/4 round bar and the pulp was eroded. The prepared cavity was washed reciprocally with 10% sodium hypochlorite and 3% hydrogen peroxide. All cavities were divided into three groups. The exposed pulps were covered with photo-crosslinkable gelatin preparation, phosphopullulan preparation and covered with conventional calcium hydroxide preparation. Then the cavities covered with photo-crosslinkable gelatin preparation were photo-irradiated. The margins of the treated areas of the molars were sealed with a protect liner. The animals were sacrificed at 1 weeks and 2 weeks after operation. In three groups, the capped treated teeth were observed at 1 and 2 weeks after operation. By 1 week after cavity preparation, the coalescence of inflammatory cells was observed subjacent to the exposed site of the dental pulp in the calcium hydroxide preparation, photo-crosslinkable gelatin preparation and phosphopullulan preparation -treated teeth. We found less inflammatory response when the cavities were treated with photo-crosslinkable gelatin preparation and phosphopullulan preparation. By 2 week after cavity preparation, the calcium hydroxide preparation and phosphopullulan preparation promote calcification. The effect of photo-curable gelatin and phosphopullulan was investigated for direct pulp capping.