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Effects of the low - level laser irradiation on arthritis

低强度激光照射对关节炎的影响

基本信息

批准号：
18592146
负责人：
OHTA Mitsuhiro
金额：
$ 2.21万
依托单位：
Nihon University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

项目摘要

The purpose of this research is to demonstrate the effect for low-level laser irradiation over the living body proving the experiment system using an animal. The pathologic model of rheumatoid arthritis was product and the effect for inflammation by low-level laser irradiation was examined by performing diode laser irradiation and FEL irradiation.We use rat as laboratory animals. The rat received a subcutaneous injection of type II collagen of the cow as an antigen to back, and the symptoms of arthritis were induced. The animal was divided into three groups as five per group, the 1st group was the control group, the 2nd group was the CIA group performing collagen sensitization which it has not laser irradiated, and the 3rd group was considered as the CIA+LLI group which performed collagen sensitization and also carried out laser irradiation every several days. The general state of the rat was observed during the experimental periods, it measured weight once per week, measured swelling of the joint portion with slide calipers, and extracted blood from the jugular vein.As a result, it was accepted that two kinds of low-level laser irradiation controls swelling of the hind foot of the rat which was caused experimentally in any case. Moreover, when the concentration in the serum of IL-1beta and IL-6 which is inflammatory cytokine were measured, as compared with the CIA group, it was falling intentionally by the CIA+LLI group. From these results, it was suggested that low-level laser irradiation has an effect which controls swelling by inflammation. Furthermore, it was suggested that low-level laser irradiation is a cure which can expect the noninvasive anti-inflammation effect without side effects.

这项研究的目的是演示弱激光照射活体的效果，并用动物来验证实验系统。制作类风湿关节炎病理模型，采用半导体激光照射和自由电子激光照射的方法，观察低强度激光照射对炎症反应的影响。大鼠皮下注射牛II型胶原作为背部抗原，诱发关节炎症状。将动物分为3组，每组5只，第1组为对照组，第2组为未经激光照射的CIA组，第3组为CIA+LLI组，即CIA+LLI组，每隔数天进行一次激光照射。在实验期间观察大鼠的一般状态，每周测量一次体重，用滑动卡尺测量关节部分的肿胀，并从颈静脉取血。结果表明，两种低强度激光照射都能控制实验中引起的大鼠后足肿胀。此外，当测定血清中炎性细胞因子IL-1β和IL-6的浓度时，与CIA组相比，CIA+LLI组有意降低。这些结果表明，低强度激光照射具有抑制炎症肿胀的作用。并认为低强度激光照射是一种无创、无副作用的抗炎治疗方法。