Effects of the low - level laser irradiation on arthritis

本研究的目的是为了证明低水平激光照射对活体的影响，证明实验系统在动物身上的应用。建立类风湿关节炎病理模型，采用二极管激光照射和自由电子激光器照射观察低强度激光照射对炎症反应的影响。我们用老鼠做实验动物。大鼠皮下注射牛ⅱ型胶原蛋白作为背部抗原，诱导关节炎症状。将动物分为三组，每组5只，第一组为对照组，第二组为CIA组，进行胶原致敏，未进行激光照射，第三组为CIA+LLI组，进行胶原致敏，每隔几天进行激光照射。实验期间观察大鼠一般状态，每周称重1次，用滑动卡尺测量关节部分肿胀程度，颈静脉抽血。因此，两种低强度激光照射在任何情况下都可以控制实验引起的大鼠后足肿胀。此外，当测量血清中炎症细胞因子il -1 β和IL-6的浓度时，与CIA组相比，CIA+LLI组有意降低。这些结果表明，低水平激光照射具有控制炎症引起的肿胀的作用。提示低强度激光照射是一种无创、无副作用的治疗方法。

The purpose of this research is to demonstrate the effect for low-level laser irradiation over the living body proving the experiment system using an animal. The pathologic model of rheumatoid arthritis was product and the effect for inflammation by low-level laser irradiation was examined by performing diode laser irradiation and FEL irradiation.We use rat as laboratory animals. The rat received a subcutaneous injection of type II collagen of the cow as an antigen to back, and the symptoms of arthritis were induced. The animal was divided into three groups as five per group, the 1st group was the control group, the 2nd group was the CIA group performing collagen sensitization which it has not laser irradiated, and the 3rd group was considered as the CIA+LLI group which performed collagen sensitization and also carried out laser irradiation every several days. The general state of the rat was observed during the experimental periods, it measured weight once per week, measured swelling of the joint portion with slide calipers, and extracted blood from the jugular vein.As a result, it was accepted that two kinds of low-level laser irradiation controls swelling of the hind foot of the rat which was caused experimentally in any case. Moreover, when the concentration in the serum of IL-1beta and IL-6 which is inflammatory cytokine were measured, as compared with the CIA group, it was falling intentionally by the CIA+LLI group. From these results, it was suggested that low-level laser irradiation has an effect which controls swelling by inflammation. Furthermore, it was suggested that low-level laser irradiation is a cure which can expect the noninvasive anti-inflammation effect without side effects.