Effects of Low-Level Laser Irradiation in Cultured Synovial Cells

低强度激光照射对培养滑膜细胞的影响

• 批准号: 15592070
• 负责人: OHTA Mitsuhiro
• 金额: $ 2.18万
• 依托单位: Nihon University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15592070/
• 关键词: Laser irradiation; Synovial cell; Cytokine

Immediate effects of low-level laser irradiation (LLLI), such as anti-inflammation and relieving pain against temporomandibular joint(TMJ) disorders, have been reported. However, the molecular based mechanisms are not elucidated yet. Synovial cells are believed to play pathological roles in the development and continuation of inflammation. Cytokine, such as Interleukin(IL)-6,8 and monocyte chemoattractant protein(MCP)-1,has been found in synovial fluid from patients with TMJ synovitis as well as IL-1β.The purpose of this study is examined the effect of LLLI on cytokines production stimulated in human synovial cells treated with IL-1β. The cells were cultured using outgrowth method. The confluent-stage cells were treated with IL-1β as the same time, LLLI was performed to the cells. The amounts of cytokines in conditioned medium were measured by ELISA kit. LLLI significantly reduced the production of IL-6, 8 and MCP-1 in early time. These findings suggest that LLLI may have anti-inflammatory effect on TMJ disorder through the reduction of cytokines production.To determine the effect of prophylactic or therapeutic treatment with low-laser irradiation on paw inflammation, bone and cartilage destruction in type II collagen-induced arthritis(CIA) model in rats. The low-level laser irradiation at prophylactic or therapeutic treatment significantly suppressed the increase in hindpaw swelling and joint destruction. In the prophylactic and therapeutic treatment group at 23 days, swelling inhibition index (%) was respectively 58.1%, 54.4%. The low-level laser irradiation suppressed radiographic changes in hindpaw.These results show that low-level laser irradiation is effective in suppressing inflammation, damage to bone and cartilage in rat CIA, and may increase efficacy of therapy for rheumatoid arthritis.

据报道，低水平激光照射（LLLI）对颞下颌关节（TMJ）疾病的直接影响，如抗炎和缓解疼痛。然而，基于分子的机制尚未阐明。滑膜细胞被认为在炎症的发展和持续中起病理作用。细胞因子，如白细胞介素(IL)-6、8和单核细胞趋化蛋白(MCP)-1，在TMJ滑膜炎患者的滑液中被发现，以及IL-1β。本研究的目的是研究LLLI对IL-1β处理的人滑膜细胞产生的细胞因子的影响。采用体外培养法培养细胞。同时用IL-1β处理融合期细胞，并对细胞进行LLLI处理。采用ELISA试剂盒检测条件培养基中细胞因子的含量。LLLI在早期显著降低IL-6、8和MCP-1的产生。这些发现表明，LLLI可能通过减少细胞因子的产生对TMJ疾病具有抗炎作用。探讨低激光对II型胶原诱导关节炎（CIA）模型大鼠足部炎症及骨、软骨破坏的预防或治疗作用。预防性或治疗性低水平激光照射均能显著抑制后肢肿胀和关节破坏的增加。预防组和治疗组23 d肿胀抑制指数（%）分别为58.1%、54.4%。低强度激光照射抑制了后爪的影像学变化。提示低强度激光照射可有效抑制大鼠CIA的炎症、骨和软骨损伤，并可能提高类风湿关节炎的治疗效果。