The effect of antianxiety agent, saliva stimulant and gastric antisecretory activity on brux-like activity in rats

本研究的目的是通过比较情绪应激组(情绪应激组)和无情绪应激组(对照组)大鼠的脑电波、咀嚼肌活动和Brux样活性表达频率，探讨情绪应激与Brux样活性的关系。然而，通过脑电波自动研究大鼠的睡眠阶段还没有建立起来。我们通过对人类脑波数据的调查，检验了现有时序数据分析软件的有效性，这些数据已经由科学操作员分析过，他们可以确定睡眠阶段。结果表明，该时序数据分析软件具有较高的准确度，可以自动判断睡眠深度和睡眠阶段。其次，在7~8周龄Wister大鼠全麻下，将电动螺丝钉插入颅骨，并用铜线连接。然后，通过左侧咬肌将针电极插入颅骨顶区。这些铜线通过不断嵌入的微型连接器连接，并固定在顶区。使用该装置进行脑波、咬肌活动和眼电检查，并将导管插入颈外静脉，经皮肤固定在夹克背部。然后，研究了情绪应激时Brux样活动的频率及其变化，结果表明情绪应激时睡眠深度发生变化，Brux样活动频率增加。提示情绪应激前应用拮抗剂可抑制Brux样活性的增加。

The aim of the present study was to investigate the relationship between the emotional stress and brux-like activity by comparison of the brain wave, activity of the masticatory muscles and expression frequency of the brux-like activity of rats those with emotional stress (emotional stress group) and without emotional stress (control group).First, it was essential to investigate the sleep stage such as arousal and REM sleep stage. However, it has never been established to investigate the sleep stage of the rats automatically by the brain wave. We examined the effectiveness of the existing chronological data analysis software by investigation of the human brain wave data that has been already analyzed by the scientific operator who can determine the sleep stage. In the result, it was certified that the chronological data analysis software was useful to determine the depth and stage of the sleep automatically with high degree of accuracy.Second, electrical pole screw was inserted to cranial bone of the 7-8 week-old Wister rats under general anesthesia and it was connected with copper line. Then, a needle electrode was inserted to the parietal region of the cranial bone through left side masseter muscle. These copper lines were connected with constantly embedded micro connecter and fixed to the parietal region. Brain wave, masseter muscle activity and electro-oculogram were developed using these devices.Additionally, a catheter was inserted into external jugular vein and fixed to dorsal region of jacket through skin. Then, frequency and its change of brux-like activity were investigated when emotional stress was applied.In the result, it was suggested that depth of sleep showed changes and the frequency of the brux-like activity increased when the emotional stress was applied. It was also suggested that premedication of antagonist drug to the emotional stress controled the increase of the brux-like activity.