The effect of antianxiety agent, saliva stimulant and gastric antisecretory activity on brux-like activity in rats

抗焦虑剂、唾液刺激剂和胃抗分泌活性对大鼠磨牙样活性的影响

• 批准号: 18592249
• 负责人: NAGATA Junko
• 金额: $ 2.62万
• 依托单位: Kagoshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18592249/
• 关键词: rat; brux-like activity; emotional storess; sleep stage; electromyogram; electroencephalogram; 抗不安薬; 唾液分泌促進剤; 消化管; ストレス; ブラキシズム; 睡眠; 咀嚼筋

The aim of the present study was to investigate the relationship between the emotional stress and brux-like activity by comparison of the brain wave, activity of the masticatory muscles and expression frequency of the brux-like activity of rats those with emotional stress (emotional stress group) and without emotional stress (control group).First, it was essential to investigate the sleep stage such as arousal and REM sleep stage. However, it has never been established to investigate the sleep stage of the rats automatically by the brain wave. We examined the effectiveness of the existing chronological data analysis software by investigation of the human brain wave data that has been already analyzed by the scientific operator who can determine the sleep stage. In the result, it was certified that the chronological data analysis software was useful to determine the depth and stage of the sleep automatically with high degree of accuracy.Second, electrical pole screw was inserted to cranial bone of the 7-8 week-old Wister rats under general anesthesia and it was connected with copper line. Then, a needle electrode was inserted to the parietal region of the cranial bone through left side masseter muscle. These copper lines were connected with constantly embedded micro connecter and fixed to the parietal region. Brain wave, masseter muscle activity and electro-oculogram were developed using these devices.Additionally, a catheter was inserted into external jugular vein and fixed to dorsal region of jacket through skin. Then, frequency and its change of brux-like activity were investigated when emotional stress was applied.In the result, it was suggested that depth of sleep showed changes and the frequency of the brux-like activity increased when the emotional stress was applied. It was also suggested that premedication of antagonist drug to the emotional stress controled the increase of the brux-like activity.

本研究旨在通过比较情绪应激（情绪应激组）和非情绪应激（对照组）大鼠的脑电波、咀嚼肌活动和磨牙样活动表达频率，探讨情绪应激与磨牙样活动的关系。首先，对觉醒和REM睡眠等睡眠阶段进行研究。然而，通过脑电波自动研究大鼠的睡眠阶段还没有建立起来。我们通过调查已经由能够确定睡眠阶段的科学操作员分析的人类脑电波数据来检查现有时序数据分析软件的有效性。实验结果表明，时序数据分析软件能够自动、准确地判断睡眠的深度和阶段。第二，在全麻下将电极螺钉插入7-8周龄Wister大鼠颅骨，并与铜线连接。然后，通过左侧咬肌将针电极插入颅骨的顶骨区域。这些铜线与不断嵌入的微型连接器连接，并固定在顶骨区域。通过颈外静脉穿刺置管，经皮固定于外套背部，记录脑电、咬肌活动和眼电。然后，研究了情绪应激时磨牙样活动的频率及其变化，结果表明，情绪应激时睡眠深度发生变化，磨牙样活动频率增加。提示情绪应激前给予拮抗剂可抑制磨牙样活动的增加。

项目成果

Leptin and des-acyl ghrelin : Their role in physiological body weight regulation and in the pathological state.

瘦素和去酰基生长素释放肽：它们在生理体重调节和病理状态中的作用。

• 发表时间: 2006
• 作者: Togawa R;Nagata J;Ohmure H;Sakaguchi K;Nagayama K;Taira K;Mtsuda Y;Setoguchi T;Shimotabira T;Matsumoto J;Yamamoto T;Izumi Y;Fukunaga T;Miyawaki S;Perboni S
• 通讯作者: Perboni S

Increased circulating cholecystokinin contributes to anorexia and anxiety behavior in mice overxppeessing pancreatic polypeptide.

循环胆囊收缩素的增加导致过度分泌胰多肽的小鼠出现厌食和焦虑行为。

• 发表时间: 2007
• 期刊: Regul Pept 141
• 作者: Suda T;Nagasawa T;Kiji M;Yashiro Y;Kobayashi H;Ishikawa I;Izumi Y;宮脇正一;Ueno N
• 通讯作者: Ueno N

Leptin transgene expression in the hypothalamus enforces euglycemia in diabetic, insulin-deficient nonobese Akita mice and leptin-deficient obese ob/ob mice.

下丘脑中的瘦素转基因表达可增强糖尿病、胰岛素缺乏的非肥胖秋田小鼠和瘦素缺乏的肥胖 ob/ob 小鼠的血糖正常。

• 发表时间: 2006
• 期刊: Peptides 27(9)
• 作者: Ueno N;Inui A;Kalra PS;Kalra SP
• 通讯作者: Kalra SP

Gastrointesutinal hormones (anorexigenic peptideYY and orexigenic ghrelin) influence neural tube development.

胃肠激素（致食欲肽 YY 和致食欲生长素释放肽）影响神经管发育。

• 发表时间: 2007
• 期刊: FASEB J 21
• 作者: Yuzuriha H;Inui A;Asakawa A;Ueno N;Kasuga M;Meguid M;Miyazaki J;Ninomiya M;Herzog H;Fujimiya M
• 通讯作者: Fujimiya M

Gastro-esophageal reflux symptoms in adult patients with skeletal Class III malocclusion as examined by questionnaire.

通过调查问卷检查成年骨性Ⅲ类错牙合患者的胃食管反流症状。

• 期刊: Am J Orthod Dentofacial Orthop (In press)
• 作者: Togawa;R;Ohmure;H;Sakaguchi;K Takada H;Oikawa K;Nagata J;Yamamoto T;Tsubouchi H;Miyawaki S
• 通讯作者: Miyawaki S