The effect of antianxiety agent, saliva stimulant and gastric antisecretory activity on brux-like activity in rats

本研究通过比较情绪应激大鼠（情绪应激组）和非情绪应激大鼠（对照组）的脑电波、咀嚼肌活动和磨牙样活动的表达频率，探讨情绪应激与磨牙样活动之间的关系。首先，有必要研究睡眠阶段，如觉醒和快速眼动睡眠阶段。然而，利用脑电波自动研究大鼠睡眠阶段的方法尚未建立。我们通过调查人类脑电波数据来检验现有时间数据分析软件的有效性，这些数据已经被能够确定睡眠阶段的科学操作员分析过。结果证明，时序数据分析软件能够自动准确地确定睡眠深度和阶段。其次，在全身麻醉下7 ~ 8周龄Wister大鼠颅骨内插入电极螺钉，并用铜线连接。然后，针电极通过左侧咬肌插入颅骨顶骨区。这些铜线通过不断嵌入的微连接器连接并固定在顶骨区域。利用这些装置开发了脑电波、咬肌活动和眼电图。另外，将导管插入颈外静脉，经皮肤固定于夹克背侧。在此基础上，研究了应激作用下脑刷样活动的频率及其变化。结果表明，当施加情绪压力时，睡眠深度会发生变化，类似brux的活动频率也会增加。同时提示，在情绪应激前使用拮抗剂可控制脑磨牙样活动的增加。

The aim of the present study was to investigate the relationship between the emotional stress and brux-like activity by comparison of the brain wave, activity of the masticatory muscles and expression frequency of the brux-like activity of rats those with emotional stress (emotional stress group) and without emotional stress (control group).First, it was essential to investigate the sleep stage such as arousal and REM sleep stage. However, it has never been established to investigate the sleep stage of the rats automatically by the brain wave. We examined the effectiveness of the existing chronological data analysis software by investigation of the human brain wave data that has been already analyzed by the scientific operator who can determine the sleep stage. In the result, it was certified that the chronological data analysis software was useful to determine the depth and stage of the sleep automatically with high degree of accuracy.Second, electrical pole screw was inserted to cranial bone of the 7-8 week-old Wister rats under general anesthesia and it was connected with copper line. Then, a needle electrode was inserted to the parietal region of the cranial bone through left side masseter muscle. These copper lines were connected with constantly embedded micro connecter and fixed to the parietal region. Brain wave, masseter muscle activity and electro-oculogram were developed using these devices.Additionally, a catheter was inserted into external jugular vein and fixed to dorsal region of jacket through skin. Then, frequency and its change of brux-like activity were investigated when emotional stress was applied.In the result, it was suggested that depth of sleep showed changes and the frequency of the brux-like activity increased when the emotional stress was applied. It was also suggested that premedication of antagonist drug to the emotional stress controled the increase of the brux-like activity.