Osmoregulatory MAP kinase signal transduction pathway

渗透调节 MAP 激酶信号转导通路

• 批准号: 19107004
• 负责人: SAITO Haruo
• 金额: $ 68.06万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (S)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19107004/
• 关键词: 細胞内シグナル伝達; ストレス応答; MAPキナーゼ; 浸透圧センサー; 酵母

When exposed to environmental hyperosmolarity, the budding yeast Saccharomyces cerevisiae activates the Hog1 MAP kinase signal pathway, which governs an array of adaptive responses. In this project, we investigated how the activity of the Hog1 MAPK pathway is regulated by the two newly discovered osmosensors Hkr1 and Msb2. We also investigated the roles of the membrane anchor protein Opy2, membrane protein Sho1, and the adaptor protein Ste50 in dynamic regulation of the Hog1 MAPK pathway.

当暴露于环境高渗透压时，芽殖酵母酿酒酵母激活Hog 1 MAP激酶信号通路，该通路控制一系列适应性反应。在这个项目中，我们研究了Hog1 MAPK通路的活性是如何被两个新发现的MAPK传感器Hkr1和Msb2调节的。我们还研究了膜锚蛋白Opy 2、膜蛋白Sho 1和衔接蛋白Ste50在Hog1 MAPK通路动态调节中的作用。

项目成果

Osmosensory signal transduction in budding yeast

芽殖酵母中的渗透感应信号转导

• 发表时间: 2010
• 作者: Haruo Saito

Stimulus-specific distinctions in spatial and temporal dynamics of stress-activated protein kinase kinase kinases revealed by a fluorescence response energy transfer biosensor

荧光响应能量转移生物传感器揭示了应激激活蛋白激酶激酶的空间和时间动力学的刺激特异性差异

• 发表时间: 2009
• 期刊: Mol.Cell.Biol. 29
• 作者: Tomida;T.;Takekawa;M.;O'Grady;P.;Saito;H.
• 通讯作者: H.

Dynamic control of yeast MAP kinase network by induced association and dissociation between the Ste50 scaffold and the Opy2 membrane anchor.

• DOI: 10.1016/j.molcel.2010.09.011
• 发表时间: 2010-10
• 期刊: Molecular cell
• 影响因子: 16
• 作者: Katsuyoshi Yamamoto;K. Tatebayashi;Keiichiro Tanaka;H. Saito

Glycosylation defects activate filamentous growth Kssl MAPK and inhibit osmoregulatory Hogl MAPK.

糖基化缺陷激活丝状生长 Kssl MAPK 并抑制渗透调节 Hogl MAPK。

• 发表时间: 2009
• 期刊: The EMBO Journal 28
• 作者: Sasaki,M.;Fukada,Y.;山田耕司;Yang Hui-Yu
• 通讯作者: Yang Hui-Yu

Phosphorylated Ssk1 prevents unphosphorylated Ssk1 from activating the Ssk2 mitogen-activated protein kinase kinase kinase in the yeast high-osmolarity glycerol osmoregulatory pathway

• DOI: 10.1128/mcb.00589-08
• 发表时间: 2008-09-01
• 期刊: MOLECULAR AND CELLULAR BIOLOGY
• 影响因子: 5.3
• 作者: Horie, Tetsuro;Tatebayashi, Kazuo;Saito, Haruo
• 通讯作者: Saito, Haruo