Regulatory Mechanism of Stress-Responsive Signal Transduction Pathway in Human and Yeast Cells
人和酵母细胞应激反应信号转导途径的调控机制
基本信息
- 批准号:14086202
- 负责人:
- 金额:$ 135.94万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research on Priority Areas
- 财政年份:2002
- 资助国家:日本
- 起止时间:2002 至 2006
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
1) Regulatory mechanism of human stress-responsive signal transduction pathwayWe revealed the mechanism of MTK1 activation by the stress-inducible Gadd45 proteins. Gadd45 binds to the N-terminal region of the MTK1, induces an N-C dissociation in MTK1 molecule, and thus facilitates MTK1 dimerization that leads to MTK1 activation by auto-phosphorylation. We showed that TGF-beta activates MTK1 through induction of the expression of Gadd45. We also identified a novel docking interaction between stress-activated MAPKKK (SAP3K) and stress-activated MAPKK (SAP2K). We showed that a C-terminal region in SAP2K, termed the DVD domain, was essential for this interaction, and elucidated its role in SAP2K activation. Using the specific interaction through DVD domain, we developed a new FRET probe to visualize SAP3K activity in single living cells.2) Regulatory mechanism of yeast stress-responsive signal transduction pathway.We identified the mucin-like membrane glycoproteins Hkr1 and Msb2 as putative osmosensors in the Sho1 branch of Hog1 MAPK pathway. We found a specific docking interaction between yeast osmoregulatory Ssk2 SAP3K and Pbs2 SAP2K, and showed that the interaction is essential for specific activation of Pbs2 by Ssk2. We also analyzed the functional roles of the docking interaction between Hog 1 and its activator, the Pbs2 SAP2K, as well as that between Hog1 and its inactivator, the Ptp2 protein tyrosine phosphatase. Finally, we found that the membrane protein Sho1, the cytoplasmic protein Ste50, and the small G-protein Cdc42 serve as adaptor proteins in yeast osmoregulatory Hog1 MAPK pathway.
1)人类应激反应信号转导通路的调控机制我们揭示了应激诱导的Gadd 45蛋白激活MTK 1的机制。Gadd 45与MTK 1的N-末端区域结合,诱导MTK 1分子中的N-C解离,从而促进MTK 1二聚化,导致MTK 1通过自身磷酸化活化。我们发现TGF-β通过诱导Gadd 45的表达激活MTK 1。我们还确定了一种新的对接之间的相互作用的压力激活的MAPKKK(SAP 3 K)和压力激活的MAPKK(SAP 2K)。我们发现,在SAP 2K的C-末端区域,称为DVD结构域,是必不可少的这种相互作用,并阐明其在SAP 2K激活的作用。利用DVD结构域的特异性相互作用,我们开发了一种新的FRET探针来观察单个活细胞中SAP 3 K的活性。2)酵母应激响应信号传导途径的调节机制。我们鉴定了粘蛋白样膜糖蛋白Hkr 1和Msb 2作为Hog 1 MAPK途径Sho 1分支中推定的渗透传感器。我们发现了一个特定的对接之间的相互作用,酵母菌的调节Ssk 2 SAP 3 K和Pbs 2 SAP 2K,并表明这种相互作用是必不可少的特异性激活Pbs 2的Ssk 2。我们还分析了Hog 1与其激活剂Pbs 2 SAP 2K之间的对接相互作用以及Hog 1与其失活剂Ptp 2蛋白酪氨酸磷酸酶之间的对接相互作用的功能作用。最后,我们发现,膜蛋白Sho 1,细胞质蛋白Ste 50,和小G蛋白Cdc 42作为衔接蛋白在酵母菌的调节Hog 1 MAPK途径。
项目成果
期刊论文数量(79)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Transmembrane mucins Hkr1 and Msb2 are putative osmosensors in the SHO1 branch of yeast HOG pathway
- DOI:10.1038/sj.emboj.7601796
- 发表时间:2007-08-08
- 期刊:
- 影响因子:11.4
- 作者:Tatebayashi, Kazuo;Tanaka, Keiichiro;Saito, Haruo
- 通讯作者:Saito, Haruo
A docking site determining specificity of Pbs2 MAPKK for Ssk2/Ssk22 MAPKKKs in the yeast HOG pathway
- DOI:10.1093/emboj/cdg353
- 发表时间:2003-07-15
- 期刊:
- 影响因子:11.4
- 作者:Tatebayashi, K;Takekawa, M;Saito, H
- 通讯作者:Saito, H
Activation of MTK1/MEKK4 by GADD45 through induced N-C dissociation and dimerization-mediated trans autophosphorylaion of the MTK1 kinase domain.
GADD45 通过诱导 N-C 解离和二聚化介导的 MTK1 激酶结构域反式自磷酸化来激活 MTK1/MEKK4。
- DOI:
- 发表时间:2007
- 期刊:
- 影响因子:0
- 作者:Miyake;Zenshi
- 通讯作者:Zenshi
Smad-dependent GADD45b expression mediates delayed activation of p38 MAP kinase by TGF-b.
Smad 依赖性 GADD45b 表达介导 TGF-b 对 p38 MAP 激酶的延迟激活。
- DOI:
- 发表时间:2002
- 期刊:
- 影响因子:0
- 作者:Takekawa;M.;Tatebayashi;K.;Itoh;F.;Adachi;M.;Imai;K.;^*Saito;H.
- 通讯作者:H.
The Slnl-Ypdl-Sskl multistep phosphorelay system that regulates an osmosensing MAP kinase cascade in yeast.
Slnl-Ypdl-Sskl 多步磷酸中继系统调节酵母中的渗透感应 MAP 激酶级联。
- DOI:
- 发表时间:2003
- 期刊:
- 影响因子:0
- 作者:Saito;Haruo
- 通讯作者:Haruo
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SAITO Haruo其他文献
Administrative centralization threatens commons-owning municipal sub-unit: Property Wards (Zaisanku) in Toyota City, Japan
行政集权威胁着拥有公地的市政下级单位:日本丰田市的财产区 (Zaisanku)
- DOI:
- 发表时间:
2011 - 期刊:
- 影响因子:0
- 作者:
Toshiyuki Takano;Nobuhiko Ichihara;Takuya Shibano;Hiroshi Kamitakahara;Fumiaki Nakatsubo;浪川健治;SAITO Haruo - 通讯作者:
SAITO Haruo
The Effect of Forest Management of Secondary Coniferous forests on User's Landscape Appreciation and Psychological Restorativeness.
次生针叶林森林经营对使用者景观欣赏和心理恢复的影响。
- DOI:
- 发表时间:
2016 - 期刊:
- 影响因子:0
- 作者:
TAKAYAMA Norimasa;SAITO Haruo;FUJIWARA Akio;HORIUCHI Masahiro - 通讯作者:
HORIUCHI Masahiro
SAITO Haruo的其他文献
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{{ truncateString('SAITO Haruo', 18)}}的其他基金
The mechanism of osmo-regulatory signal transduction by interactions among membrane proteins through their TM regions
膜蛋白之间通过 TM 区域相互作用进行渗透调节信号转导的机制
- 批准号:
24247034 - 财政年份:2012
- 资助金额:
$ 135.94万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
A comparative study on excluding and accepting of gathering activities in forest
森林采集活动排除与接受的比较研究
- 批准号:
24710044 - 财政年份:2012
- 资助金额:
$ 135.94万 - 项目类别:
Grant-in-Aid for Young Scientists (B)
Development of a new local-magnetic-field measurement systemutilizing positronium as the probe
以正电子素为探针的新型局域磁场测量系统的研制
- 批准号:
23654114 - 财政年份:2011
- 资助金额:
$ 135.94万 - 项目类别:
Grant-in-Aid for Challenging Exploratory Research
Regional Myocardial Rotational Motion Analysis with Cine MRI, Cardiac MDCT, and Myocardial Tagging.
使用电影 MRI、心脏 MDCT 和心肌标记进行区域心肌旋转运动分析。
- 批准号:
22591315 - 财政年份:2010
- 资助金额:
$ 135.94万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Osmoregulatory MAP kinase signal transduction pathway
渗透调节 MAP 激酶信号转导通路
- 批准号:
19107004 - 财政年份:2007
- 资助金额:
$ 135.94万 - 项目类别:
Grant-in-Aid for Scientific Research (S)
Regulatory mechanisms of the environmental stress-responsive MAP kinase signal pathway
环境应激响应MAP激酶信号通路的调控机制
- 批准号:
14104021 - 财政年份:2002
- 资助金额:
$ 135.94万 - 项目类别:
Grant-in-Aid for Scientific Research (S)
Regulatory Mechanism of Stress-Responsive Signal Transduction Pathway
应激反应信号转导通路的调控机制
- 批准号:
14086101 - 财政年份:2002
- 资助金额:
$ 135.94万 - 项目类别:
Grant-in-Aid for Scientific Research on Priority Areas
ENTRAPMENT NEUROPATHY IN ACUTE FACIAL PARALYSIS ; CIRCURATORY DISTURBANCE AND ITS TREATMENT
急性面瘫中的卡压性神经病;
- 批准号:
07457402 - 财政年份:1995
- 资助金额:
$ 135.94万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
A study of intratemporal facial nerve entrapment palsy. -Its early diagnosis and treatment-
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- 批准号:
05671433 - 财政年份:1993
- 资助金额:
$ 135.94万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
Changes of blood supply after intracanal decompression of the facial nerve
面神经管内减压术后血供变化
- 批准号:
01480407 - 财政年份:1989
- 资助金额:
$ 135.94万 - 项目类别:
Grant-in-Aid for General Scientific Research (B)
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