Mechanisms for the dendritic formation by ADP ribosylation factor 6 and its functional significance in higher brain functions

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The aim of this study is to dissect the molecular mechanism by which ADP ribosylation factor 6 (ARF6) regulates dendritic formation. First, we found that IQ-ArfGEF/BRAG1 functions as a guanine nucleotide exchange factor for ARF6 at the postsynaptic density of excitatory synapses by interacting with the PSD-95 family and IRSp53. Second, we isolated vezatin as a novel ARF6-interacting protein by yeast two hybrid screening. Furthermore, we provided the strong evidence for the functional involvement of vezatin in the dendritic formation of cultured hippocampal neurons using the RNA interference approach. These findings suggest that vezatin regulates dendritic formation downstream of ARF6.