Functional analysis of the BRAG3-Arf6 pathway in the inhibitory synapse

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The aim of this study is to provide anatomincal evidence for the BRAG3-Arf6 pathway as a novel regulator of membrane traffic and actin cytoskeleton in the inhibitory synapse. First, we immunohistochemically demonstrated that BRAG3 localizes preferentially at postsynaptic specializations of symmetric synapses. Using yeast two-hybrid and pull down assays, we identified that BRAG3 can interact with utrophin/dystrophin and S-SCAM/MAGI-2, scaffolding proteins that localize at inhibitory synapses. Taken together, these findings suggest that BRAG3 may regulate the membrane traffic and actin cytoskeleton at inhibitory synapses through the interaction with scaffold proteins such as dystrophin and S-SCAM.