Development of a novel tumor diagnostics based on the imaging of the optically coded cancer cells

开发基于光学编码癌细胞成像的新型肿瘤诊断方法

• 批准号: 19300189
• 负责人: KAUL Sunil
• 金额: $ 12.15万
• 依托单位: National Institute of Advanced Industrial Science and Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19300189/
• 关键词: 癌; 細胞; 抗体; 内在化; モータリン; 量子ドット; 診断; デリバリー

We have generated mortalin specific antibodies that showed cell-internalizing features. These antibodies were validated as nano-carriers. Antibodies were conjugated with quantum dots, FITC and expression plasmids that were delivered to cancer cells successfully. Cancer cells were labeled with various kinds of quantum dots to generate multicolor cells for long term imaging. We have demonstrated that (1) the antibody can be used for optic tagging of cells with quantum dots, (2) the optically tagged cells can be used for long term imaging of cells and (3) the tagged cells undergo normal differentiation in vitro and in vivo.

我们已经生成了具有细胞内化特征的 Mortalin 特异性抗体。这些抗体被验证为纳米载体。抗体与量子点、FITC 和表达质粒缀合，并成功递送至癌细胞。癌细胞被各种量子点标记，以产生用于长期成像的多色细胞。我们已经证明，（1）该抗体可用于用量子点对细胞进行光学标记，（2）光学标记的细胞可用于细胞的长期成像，以及（3）标记的细胞在体外和体内进行正常分化。

项目成果

Stable and non-invasive illumination of mesenchymal stem cells by internalizing quantum dots

通过内化量子点对间充质干细胞进行稳定且非侵入性的照明

• 发表时间: 2008
• 期刊: HUMAN GENE THERAPY 20
• 作者: Y. Ohyabu;Z. Kaul;T. Yoshioka;S. Sakai;H. Mishima;T. Uemuura;S. Kaul;R. Wadwha
• 通讯作者: R. Wadwha

Intemalizing antibody-based targeted gene delivery for human cancer cells

针对人类癌细胞的基于抗体的靶向基因传递的内化

• 发表时间: 2007
• 期刊: Hum. Gene Ther 18
• 作者: Shiota;M.;Ikeda;Y.;Kaul;Z.;Itadani;J.;Kaul;S.C.;and Wadhwa;R
• 通讯作者: R

Sunil Kaul, Renu Wadhwa, モータリン抗体を用いたQuantum-dot導入による間葉系幹細胞のin vitro/in vivoラベリング

Sunil Kaul，Renu Wadhwa，使用 mortalin 抗体通过量子点引入对间充质干细胞进行体外/体内标记

• 发表时间: 2008
• 作者: 大藪淑美;Zeenia Kaul;吉岡友和;井上和貴;酒井晋介;三島初;植村寿公
• 通讯作者: 植村寿公

Use of quantum dots for in vivo imaging, cell tracking and cancer diagnostics.

使用量子点进行体内成像、细胞追踪和癌症诊断。

• 发表时间: 2007
• 作者: Kaul;Z.;Yaguchi;T.;Kaul;S.C.;Wadhwa;R
• 通讯作者: R

Stress protein mortalin a dual regulator of age diseases, cancers and neurodegeneration.

应激蛋白 mortalin 是衰老疾病、癌症和神经退行性疾病的双重调节因子。

• 发表时间: 2009
• 作者: Wadhwa;R
• 通讯作者: R