Development of a novel tumor diagnostics based on the imaging of the optically coded cancer cells

我们已经产生了显示细胞内化特征的死亡蛋白特异性抗体。这些抗体被证实为纳米载体。将抗体与量子点、FITC和表达载体偶联，成功地将其传递给癌细胞。用各种量子点标记癌细胞，生成用于长期成像的多色细胞。我们已经证明，(1)该抗体可用于量子点细胞的光学标记，(2)光学标记的细胞可用于细胞的长期成像，(3)标记的细胞在体外和体内都能正常分化。

We have generated mortalin specific antibodies that showed cell-internalizing features. These antibodies were validated as nano-carriers. Antibodies were conjugated with quantum dots, FITC and expression plasmids that were delivered to cancer cells successfully. Cancer cells were labeled with various kinds of quantum dots to generate multicolor cells for long term imaging. We have demonstrated that (1) the antibody can be used for optic tagging of cells with quantum dots, (2) the optically tagged cells can be used for long term imaging of cells and (3) the tagged cells undergo normal differentiation in vitro and in vivo.