Developmental and Evolutionary Analysis of Ancient Fish (Polypterus)

古代鱼类（Polypterus）的发育和进化分析

• 批准号: 19310125
• 负责人: NARUSE Kiyoshi
• 金额: $ 12.9万
• 依托单位: National Institute for Basic Biology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19310125/
• 关键词: ゲノム進化・再編; 古代魚; ゲノム進化; 条鰭類; 肉鰭類; ゲノム重複; ポリプテルスセネガルス; 肺発生カスケード; エンハンサー

Establishment of cultured cell line : We have been established the primary cell culture from fin, kidney and embryos of Polypterus senegalus. Initial phase of cell culture, cells were growing well. After several passages, most of cells were rabidly dying by apoptosis. This phenomenon was observed in fins, kidney and embryo. Although we have changed several culture conditions such as medium and collagen coated dish, all efforts were not successful.Construction of fosmid library and evolutionary genetic analysis : We have constructed the Polypterus genomic library with fosmid vector system. Independent colony members of this library reached over 500,000. After PCR screening of three genes (Gcm2、Elovl5、Fbox93), single positive clone with the Elovl5 gene was identified. This result suggests the genome coverage of this fosmid library was not high enough for isolation of arbitral genes in Polypterus genome.

培养细胞系的建立：我们已经建立了紫背鱼鳍、肾和胚胎的原代细胞培养。细胞培养初期，细胞生长良好。经过几代传代后，大多数细胞因细胞凋亡而狂暴死亡。在鳍、肾和胚胎中观察到这种现象。虽然我们改变了几种培养条件，如培养基和胶原包被盘，但都没有成功。Fosmid文库的构建和进化遗传分析：我们用Fosid载体系统构建了多翅目昆虫基因组文库。这个图书馆的独立群体成员达到了50多万人。对3个基因(Gcm2、Elov15、Fbox、93)进行聚合酶链式反应筛选，获得1个含有Elov5基因的阳性克隆。这一结果表明，该文库的基因组覆盖率不够高，不足以分离多翅目昆虫基因组中的任一基因。

项目成果

Whole-Mount In Situ Hybridization of Bichir (Polypterus) Embryos

Bichir（Polypterus）胚胎的整体原位杂交

• 发表时间: 2009
• 作者: Takeuchi M;Okabe M;Aizawa S
• 通讯作者: Aizawa S

Microinjection of Bichir (Polypterus) Embryos

Bichir（Polypterus）胚胎显微注射

• 发表时间: 2009
• 作者: Takeuchi M;Okabe M;Aizawa S
• 通讯作者: Aizawa S

呼吸器の構造進化～脊椎動物の上陸の前後～

呼吸器官的结构演化：脊椎动物登陆前后

• 发表时间: 2010
• 作者: Kasahara;M.;Naruse;K.;et. al.;岡部正隆
• 通讯作者: 岡部正隆

The medaka draft genome and insights into vertebrate genome evolution

• DOI: 10.1038/nature05846
• 发表时间: 2007-06-07
• 期刊: NATURE
• 影响因子: 64.8
• 作者: Kasahara, Masahiro;Naruse, Kiyoshi;Kohara, Yuji
• 通讯作者: Kohara, Yuji

Integration of human mesenchymal stem cells into the Wolffian duct in chicken embryos

• DOI: 10.1016/j.bbrc.2009.05.052
• 发表时间: 2009-07-31
• 期刊: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
• 影响因子: 3.1
• 作者: Fukui, Akira;Yokoo, Takashi;Okabe, Masataka
• 通讯作者: Okabe, Masataka