Metal Ion Specificity, Four-Electron Reduction of Dioxygen, and Creation of Functions of a Multicopper Oxidase Involved in Detoxification of Copper
Metal Ion Specificity, Four-Electron Reduction of Dioxygen, and Creation of Functions of a Multicopper Oxidase Involved in Detoxification of Copper
批准号:
19350081
负责人:
SAKURAI Takeshi
金额:
$11.98万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (B)
财政年份:
2007
资助国家:
日本
项目状态:
已结题
起止时间:
2007 至 2009
中文摘要
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英文摘要
銅排出酵素系において特異的にCu(I)を酸化する酵素CueOから50残基のアミノ酸を除去し,有機基質に対する酸化活性を創製するとともに,触媒部位への配位アミノ酸への変異導入や水素結合の切断や形成によって、触媒機能を高めることに成功した。また,CueOの組換え体および変異体を,生物燃料電池のカソード触媒やヘアカラー色素作成酵素としての利用することを可能とした。さらに,変異体の反応によって,酸素の4電子還元機構を明らかにし,反応に関与する非配位性の酸性アミノ酸を特定した。
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一価銅オキシダーゼCueOの水素結合による酵素活性制御
通过单价铜氧化酶 CueO 的氢键调节酶活性
DOI:
--
发表时间:
2009
期刊:
影响因子:
--
作者:
[小木裕貴, 前田康雄, 片岡邦重, 瀬尾悌介, 櫻井武]
通讯作者:
櫻井武
Reduction of Dioxygen by a Multicopper Oxidase, CueO, and Roles of Asp112 and Glu506 Located Adjacent to the Trinuclear Copper Center
多铜氧化酶 CueO 还原分子氧以及位于三核铜中心附近的 Asp112 和 Glu506 的作用
DOI:
--
发表时间:
2009
期刊:
影响因子:
--
作者:
[Sakurai, T., Kataoka, K., Sugiyama, R., Hirota, S., Inoue, M., Urata, K., Minagawa, Y., Seo, D.]
通讯作者:
D.
マルチ銅オキシダーゼCueO変異体による電気化学的酸素還元反応の解析
多铜氧化酶CueO突变体电化学氧还原反应分析
DOI:
--
发表时间:
2007
期刊:
影响因子:
--
作者:
[三浦侑子, 黒瀬伸治, 上高雄二, 片岡邦重, 櫻井武, 辻村清也, 加納健司]
通讯作者:
加納健司
Wiley, Copper Oxygen Chemistry
威利,铜氧化学
DOI:
--
发表时间:
2010
期刊:
影响因子:
--
作者:
[Multicopper Proteins, Sakurai, T., Kataoka, K]
通讯作者:
K
マルチ銅オキシダーゼの酸素4電子還元反応の解析
多铜氧化酶氧四电子还原反应的分析
DOI:
--
发表时间:
2007
期刊:
影响因子:
--
作者:
[杉山良輔, 浦田香苗, 井上めぐみ, 瀬尾悌介, 片岡邦重, 櫻井武]
通讯作者:
櫻井武
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