Nonlinear Raman microscopy to detect membrane protein

我们利用微透镜阵列扫描器研制了一台用于实时分子成像的多焦点激发非线性拉曼显微镜。用来自两个高度受控的PS锁模激光(抖动为30fs，逐点波长扫描在300ms内)的光平行曝光样品，并使用图像传感器进行并行检测，从而实现实时成像。我们演示了活细胞的实时拉曼成像(帧速率为10fps)。我们还将所研制的系统与激光消融相结合，对细胞膜进行了破坏，并演示了细胞膜修复过程。我们在非线性拉曼显微镜系统中加入了偏振模式控制系统，通过控制聚焦光斑上的电磁场方向来检测膜蛋白。液晶成像证实了对有序分子进行选择性检测的可能性。

We developed a multi-focus excitation nonlinear Raman microscope using a microlens array scanner for real-time molecular imaging. Parallel exposure of a specimen with light from two highly controlled ps mode-locked lasers (jitter of 30fs, point-by-point wavelength scan within 300ms) and parallel detection with an image sensor enabled real-time imaging. We demonstrated real-time Raman imaging of living cells (frame rate of 10fps). We also combined the developed system with the laser ablation to destruct the cell membrane, and demonstrated to observe the membrane repairing process. We added the polarization pattern control system to a nonlinear Raman microscopy system to detect membrane proteins by control the direction of electro-magnetic filed on the focus spot. The possibility of the selective detection of ordered molecules was confirmed with the imaging of liquid crystal.