Utilization of protoplast for producing and screening of useful metabolites and it's application.

点击翻译按钮获取中文摘要

A high-speed system for production of useful materials using protoplasts was developed. In microbial and plant protoplasts, some products are released freely into the broth with the double consequences of increasing overall productivity and facilitating down stream processing. Furthermore, since many high molecular weight substances which are otherwise not accessible to the cells (due to the presence of cell wall) can directly access the cell membrane in protoplasts, the composition and concentrations of the metabolites produced by protoplasts vary from those produced by cells. It was found that the yeast protoplasts are cultured in a liquid static culture so as to diffuse cell wall components in the culture broth with the result that while maintaining the state of the protoplasts, protoplasts could produce the cell wall components in the culture broth. By optimizing the culture conditions, large amount of the cell wall components with antitumor activity was produced. Taxus protoplasts with artificial cell walls produce taxiods efficiently and released the compounds into the medium. Moreover, the adequate supply of methyl-jasmonate, etc. were useful to enhance the taxoids production in Taxus protoplasts. This novel system has great potential in useful metabolite production.