Study on molecular carcinogenesis in transient amplifying cell of human prostate

人前列腺瞬时扩增细胞分子致癌作用的研究

• 批准号: 19390104
• 负责人: KONISHI Noboru
• 金额: $ 6.24万
• 依托单位: Nara Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19390104/
• 关键词: 前立腺; 癌化; 組織幹細胞; senescence; transient amplifying cell; junB; tranint amlifying cell

Transient amplifying (TA) cells are a subset of basal cell populations within the prostate from which cancers are thought to originate. Most TA cell populations showed increased expression of p53, p21, p16, and pRb, resulting in senescence. However, TA cell clones with reduced p16 expression successfully bypassed this phase. The close correlation was found between the levels of junB and p16 expression. Transfection of junB siRNA in prostatic TA cells allowed the cells to escape senescence, presumably through inactivation of p16/pRb ; this suggests that activation of junB/p16/pRb is required to block clonal expansion. Interestingly, the percentage of peripheral zone TA cells evading senescence was significantly higher than those from central and transition zones. Metastatic prostate tumors, as well as prostate cancers with high Gleason scores, demonstrated significantly low junB immunopositivity. junB thus apparently plays an important role in controlling prostate carcinogenesis and may be a new target for cancer prevention and therapy.

瞬时扩增（TA）细胞是前列腺内基底细胞群的一个子集，癌症被认为起源于前列腺。大多数TA细胞群体显示p53、p21、p16和pRb表达增加，导致衰老。然而，具有降低的p16表达的TA细胞克隆成功地绕过了该阶段。junB与p16表达水平密切相关。在前列腺TA细胞中转染junB siRNA允许细胞逃避衰老，推测是通过p16/pRb的失活;这表明需要激活junB/p16/pRb来阻断克隆扩增。有趣的是，周围区TA细胞逃避衰老的百分比显着高于中央和过渡区。转移性前列腺肿瘤，以及前列腺癌与高Gleason评分，表现出显着低junB免疫阳性。junB在前列腺癌的发生发展中起重要作用，可能成为前列腺癌预防和治疗的新靶点。

项目成果

DNA microarray analysis of multicentric prostate cancer based on the computer-assisted three-dimensional model

基于计算机辅助三维模型的多中心前列腺癌DNA微阵列分析

• 发表时间: 2008
• 作者: Ishida;E.;Nakamura;M.;Shimada;K.;Tasaki;M.;Konishi;N.
• 通讯作者: N.

前立腺progenitor cellの生物学的特性と病理組織学的意義について

前列腺祖细胞的生物学特性及组织病理学意义

• 发表时间: 2008
• 作者: 島田啓司;中村光利;石田英和;樋口智紀;小西登
• 通讯作者: 小西登

Clinicopathological diagnosis of gliomas by genotype analysis

神经胶质瘤的基因型分析临床病理诊断

• 发表时间: 2009
• 期刊: Brain Nerve 61
• 作者: Nakamura;M.;Shimada;K.;et al
• 通讯作者: et al

Analysis of the genes associated with premature senescence in prostate cancer and stromal cells of treated cases

前列腺癌及治疗病例基质细胞早衰相关基因分析

• 发表时间: 2009
• 作者: Ishida;E.;Shimada;K.;et al
• 通讯作者: et al

非小細胞肺癌におけるhABHファミリーの発現

hABH家族在非小细胞肺癌中的表达

• 发表时间: 2009
• 作者: 田崎正人;島田啓司;他
• 通讯作者: 他