Molecular physiological analyses of renal failure in pediatric cystic renal diseases

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Among renal diseases in childhood, the rate of hereditary renal diseases such as cystic kidneys has been increased. To promote the analyses of these diseases, we conducted a series of studies including long-term in vitro microperfusion of renal tubules from normal mouse kidneys. The physiological properties of mouse cortical thick ascending limbs of Henle's loop including appearance and transepithelial voltages were not affected by 48 hours long-microperfused preparation, indicating that this new technique is applicable to many types of investigations for adaptation both rapid and slow of renal tubules to various experimental manipulations.It has also been demonstrated the cortical thick ascending limbs of Henle's loop in PCK rat kidneys are as sensitive to vasopressin as those of tubules from normal SD rats.Although we lost major parts of our research data due to disaster in March 2011 in Japan, our present research experiences strongly suggest that long-term in vitro microperfusion of renal tubules will give us important information on the physiology and pathophysiology of renal cystic diseases further.