Engineering three dimensional hepatic tissue with microvascular networks in vivo

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Creation of the highly functional three dimensional (3-D) tissue with microvascular networks has been eagerly waited for regenerative medicine, however, the technology of such reconstitution is not established enough. The final goal of our study is creation of human highly functional and complicated tissues. To reach this final goal, we first seeded human umbilical-vein endothelial cells (HUVECs) and human mesenchymal stem cells (hMSC) in a three-dimensional fibronectin-collagen gel and reconstituted human 3D microvessels in vitro. Next, we reconstituted this human microvessls in vivo by transplanting a small piece of this fibronectin-collagen gel embedded HUVEC and hMSC in mouse cranial windows and aimed at the creation of human 3D hepatic tissue with microvascular network.