In vivo imaging of liver organogenesis from human liver bud

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In this study, we aimed to establish a visualization system to monitor liver organogenesis from liver bud in vivo. To this end, we developed a liver bud generation method according to the three-dimensional co-culture of human liver stem cells, human umbilical vein endothelial cells (HUVEC) and human mesenchymal stem cells (hMSC). Next, we established a 4D imaging system using ultra-high-speed confocal microscope system. Then we transplanted the human liver bud into the cranial window and observed visualized and reconstituted human liver tissue with human vascular network. We have established an imaging technology to visualize and analyze the formation of human solid organs.