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The development of noninvasive and economical method to evaluate the bone quality using the measurement of serum levels of osteocyte-specific-proteins

开发通过测量骨细胞特异性蛋白血清水平来评估骨质量的无创且经济的方法

基本信息

批准号：
19390393
负责人：
HASHIMOTO Jun
金额：
$ 11.9万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2007
资助国家：
日本
起止时间：
2007 至 2009
项目状态：
已结题

项目摘要

It is revealed by our preliminary analysis that serum level of matrix extracellular phosphoglycoprotein (MEPE) that is one of osteocyte-specific-proteins is correlated with the multiplicity of osteoporotic vertebral fracture. However, the amino sequence analysis of MEPE derived fragment present in serum using monoclonal antibodies against MEPE revealed that mAb also bind with the unknown peptide derived from some protein other than MEPE. Identification of this foreign peptide is unfortunately too difficult due to its small amount and the cross reactivity itself in mAb, so clinical application of ELISA using mAb against MEPE has not been established. However, our in vitro study confirmed that the possible importance of the measurement of osteocyte-specific protein and showed the next target protein for development of clinically applicable plasma concentration measurement.

初步分析表明，血清基质细胞外磷酸糖蛋白(MEPE)是骨细胞特异性蛋白之一，与骨质疏松性椎体骨折的多发性有关。然而，用抗MEPE的单抗对血清中存在的MEPE衍生片段进行的氨基酸序列分析表明，mAb也与MEPE以外的其他蛋白质衍生的未知肽结合。遗憾的是，由于这种外源多肽的量很少，而且在单抗中存在交叉反应，鉴定起来太困难了，所以利用单抗进行抗MEPE的ELISA法尚未建立临床应用。然而，我们的体外研究证实了骨细胞特异性蛋白测量的可能重要性，并显示了下一个可用于临床应用的血药浓度测量的靶蛋白。

项目成果

期刊论文数量（0）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

In Vitro Generation of a Scaffold-Free Tissue-Engineered Construct(TEC)Derived from Human Synovia1 Mesenchyma1 stem Ce11s : Biological and Mechanical Properties, and Further Chondrogenic Potential.

源自人类滑液1间充质1干细胞Ce11s的无支架组织工程结构（TEC）的体外生成：生物和机械特性，以及进一步的软骨形成潜力。

DOI：
发表时间：
2008
期刊：
Tissue Eng Part A 14
影响因子：
0
作者：
Ando W;Tateishi K;Katakai D;Hart DA;Higuchi C;Nakata K;Hashimoto J;Fujie H;Shino K;Yoshikawa H. Nakamura N.
通讯作者：
Yoshikawa H. Nakamura N.

A combination of biochemical markers of cartilage and bone turnover, radiographic damage and body mass index to predict the progression of joint destruction in patients with rheumatoid arthritis treated with disease-modifying anti-rheumatic drugs