Implication of Oxygen Tension in Bone Metabolism

我们发现，在支持细胞存在的情况下，高氧张力显著延长破骨细胞前体形成的时间，并显著持续增加支持细胞产生巨噬细胞集落刺激因子（M-CSF）。这些发现可以部分解释严重骨破坏或骨溶解伴丰富血管和大量破骨细胞的病理生理现象，如类风湿关节炎（RA）和原发性或转移性肿瘤。我们还发现，在氧气浓度为20%和5%的情况下，o2浓度随深度的变化而降低，因此介质深度是研究细胞周围氧张力与细胞生物学关系的关键条件之一。

We showed that a high oxygen tension markedly prolonged the duration of osteoclast precursor formation in the presence of supporting cells, and also markedly and persistently increased the production of macrophage colony stimulating factor (M-CSF) by supporting cells. These findings could explain some part of pathophysiology in severe bone destruction or osteolysis accompanied with abundant blood vessel and numeraous osteoclasts seen in several pathological conditions such as rheumatoid arthritis (RA) and primary or metastatic tumors. We also showed that O_2 concentrations decreased depending of the depth in both 20 and 5% O_2 tension, so medium depth is one of critical conditions to study the relation of the cellular environment of pericellular oxygen tension and cell biology.