Implication of Oxygen Tension in Bone Metabolism

氧张力对骨代谢的影响

• 批准号: 20591777
• 负责人: HASHIMOTO Jun
• 金额: $ 2.58万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20591777/
• 关键词: 骨・軟骨代謝学; 低酸素濃度; 骨形成; 軟骨分化; 低酸素; Nkx3.2; 転写因子; 破骨細胞; 高酸素濃度; 酸化ストレス; M-CSF; 生存

We showed that a high oxygen tension markedly prolonged the duration of osteoclast precursor formation in the presence of supporting cells, and also markedly and persistently increased the production of macrophage colony stimulating factor (M-CSF) by supporting cells. These findings could explain some part of pathophysiology in severe bone destruction or osteolysis accompanied with abundant blood vessel and numeraous osteoclasts seen in several pathological conditions such as rheumatoid arthritis (RA) and primary or metastatic tumors. We also showed that O_2 concentrations decreased depending of the depth in both 20 and 5% O_2 tension, so medium depth is one of critical conditions to study the relation of the cellular environment of pericellular oxygen tension and cell biology.

我们发现，高氧张力显着延长破骨细胞前体形成的持续时间在支持细胞的存在下，也显着和持续增加巨噬细胞集落刺激因子（M-CSF）的支持细胞的生产。这些发现可以解释某些病理条件下，如类风湿性关节炎（RA）和原发性或转移性肿瘤中出现的严重骨破坏或骨溶解，伴随着丰富的血管和大量的破骨细胞。在20%O_2和5%O_2条件下，O_2浓度随深度的增加而降低，因此，中等深度是研究细胞周围氧分压与细胞生物学关系的关键条件之一。

项目成果

A combination of biochemical markers of cartilage and bone turnover, radiographic damage and body mass index to predict the progression of joint destruction in patients with rheumatoid arthritis treated with disease-modifying anti-rheumatic drugs

• DOI: 10.1007/s10165-009-0170-4
• 发表时间: 2009-06-01
• 期刊: MODERN RHEUMATOLOGY
• 影响因子: 2.2
• 作者: Hashimoto, Jun;Garnero, Patrick;Nishimoto, Norihiro
• 通讯作者: Nishimoto, Norihiro

High oxygen tension prolongs the survival of osteoclast precursors via macrophage colony-stimulating factor Bone.

高氧张力通过巨噬细胞集落刺激因子骨延长破骨细胞前体的存活。

• 发表时间: 2009
• 期刊: Bone 44(1)
• 作者: Yamasaki N;Tsuboi H;Hirao M;Nampei A;Yoshikawa H;Hashimoto J.
• 通讯作者: Hashimoto J.

Adenovirus-mediated gene transfer of adiponectin reduces the severity of collagen-induced arthritis in mice

• DOI: 10.1016/j.bbrc.2008.11.005
• 发表时间: 2009-01-09
• 期刊: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
• 影响因子: 3.1
• 作者: Ebina, Kosuke;Shima, Kazuya;Shimomura, Iichiro
• 通讯作者: Shimomura, Iichiro

Laboratory and febrile features after joint surgery in rheumatoid arthritis patients treated with tocilizumab.

使用托珠单抗治疗的类风湿关节炎患者关节手术后的实验室和发热特征。

• 发表时间: 2009
• 期刊: Ann Rheum Dis. 68(5)
• 作者: Hirao M;Hashimoto J;Tsuboi H;Nampei A;Nakahara H;Yoshio N;Mima T;Yoshikawa H;Nishimoto N.
• 通讯作者: Nishimoto N.

Pericellular oxygen concentration in osteoblast culture

成骨细胞培养物中细胞周氧浓度

• 发表时间: 2010
• 作者: 小瀬弘樹、橋本淳;他
• 通讯作者: 他