The role of PPAR in metabolic syndrome with novel genetically modified rabbits

PPAR 在新型转基因兔代谢综合征中的作用

• 批准号: 19500362
• 负责人: KITAJIMA Shuji
• 金额: $ 2.58万
• 依托单位: Saga University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19500362/
• 关键词: 遺伝子組換え; ウサギ; 肥満; 病態モデル; PPAR

We generated transgenic (Tg) rabbits as a novel human disease model for metabolic syndrome. PPAR-gamma has been believed to be associated with pathologic status of it. Tg rabbits was designed to specifically overexpress human PPAR-gamma in their adipose tissue. As the results, we confirmed specific expression of mRNA of transgene in the adipose tissue of Tg rabbits, however, we could not find phenotypic differences (pathologic status) between Tg rabbits and wild type one. It is thought that more detailed further examination such as manifestation protein activity is required in future.

我们建立了转基因(TG)兔作为一种新的代谢综合征的人类疾病模型。PPAR-γ被认为与其病理状态有关。转基因兔的设计目的是在它们的脂肪组织中特异性地过表达人PPAR-γ。结果证实转基因基因在转基因兔脂肪组织中有特异性表达，但未发现转基因兔与野生型转基因兔的表型差异(病理状态)。认为未来还需要更详细的进一步检查，如表现蛋白活性。

项目成果

Re-establishment of Complement C6-deficeint Rabbit Colony by Cryopreserved Sperm Transported from Abroad.

利用国外运输的冷冻精子重建补体 C6 缺陷兔群体。

• 发表时间: 2007
• 期刊: Exp. Anim 55
• 作者: 金城 貴夫;岩政 輝男;Liu E.et al.
• 通讯作者: Liu E.et al.

ウサギの血清分離方法の検討

兔血清分离方法的探讨

• 发表时间: 2009
• 作者: 渡辺賢;小比類巻生;湯本正寿;漢那宗士
• 通讯作者: 漢那宗士

Increased expression of vascular endothelial growth factor in kidney leads to progressive impairment of glomerular functions

• DOI: 10.1681/asn.2006010075
• 发表时间: 2007-07-01
• 期刊: JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
• 影响因子: 13.6
• 作者: Liu, Enqi;Morimoto, Masatoshi;Fan, Jianglin
• 通讯作者: Fan, Jianglin

Matrix metalloproteinase 12 is a factor that accelerates the initiation of atheroscle rosis and stimulates the progression of fatty streaks to fibrous plaques in transgenic rabbits

基质金属蛋白酶 12 是一种加速转基因兔动脉粥样硬化发生并刺激脂肪纹发展为纤维斑块的因子

• 发表时间: 2008
• 期刊: Am J Pathol 172
• 作者: Yamada S;et. al.
• 通讯作者: et. al.

Temporal and quantitative analysis of expression of metalloproteinase (MMPs) and their endogenous inhibitors in atherosclerotic lesions.

动脉粥样硬化病变中金属蛋白酶（MMP）及其内源性抑制剂表达的时间和定量分析。

• 发表时间: 2008
• 期刊: Histol. Histopathol. 23
• 作者: Yu;Y.;Koike;T.;Kitajima;S.;Liu;E.;Morimoto;M.;Shiomi;M.;Hatakeyama;K.;Asada;Y.;Wang;K-Y.;Sasaguri;Y.;Watanabe;T.;Fan;J.
• 通讯作者: J.