Quantitative Evaluation of myofilaments remodeling during relaxation-contraction cycling in smooth muscle cells.

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To quantitatively evaluate the relation between myofilament remodeling and mechanical properties in vertebrate smooth muscle cells, by using electron microscopy, florescent resonance energy transfer (FRET) analysis and conventional electrophoresis techniques, we measured both the contractile force and changes in the myofilament structure of the same preparations of skinned (cell membrane permeabilized) taenia cecum. Also an X-ray diffraction study using skinned taenia cecum showed dynamic changes in the lattice structure originated from myofilament arrangement during contraction-relaxation cycles.