Cellular immunological activities of antigenic N-glycans from unused seaweed-glycoproteins and it's applications.

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In this study, we analyzed the structures and cellular immunological activities of N-glycans linked to seaweed glycoproteins. First, glycopeptides were prepared from the pepsin-digests of seaweed homogenates (brown algae, red seaweeds, and green laver) by gelfiltration and HPLC. Glycan moieties were liberated by hydrazinolysis and then were fluorescence-labeled. The structural analysis using HPLC-mapping showed that major N-glycans of hydrophytes are typical high-mannose type structures, while the typical plant complex type N-glycans having α1-3Fuc and β1-2 Xyl residues were not found. However, we found some unique glycans that were resistant to several glycosidases, indicating that some hydrophytes-specific glycans might occur on some seaweed glycoproteins. Furthermore, the immunological activity of the seaweed glycopeptides was assayed, and it was found that some glycopeptide-fraction could stimulate human monocytes.