Studies on the formation of basic ground of tideland ecosystem from the viewpoints of sediment protease activities.

从沉积物蛋白酶活性角度研究滩涂生态系统基础的形成。

• 批准号: 19510012
• 负责人: NAKAMURA Kenichi
• 金额: $ 2.58万
• 依托单位: Prefectural University of Hiroshima
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19510012/
• 关键词: 河口干潟; プロテアーゼ; 有機物質負荷; 窒素代謝循環; 干潟生態系; 電気泳動法; 有機物負荷; 干潟; 微生物; 分泌酵素; 植物プランクトン; 窒素循環; 底質; 生態系

In tideland sediments, microorganisms secrete extracellular enzymes, which support waste purification and nutrient supplementation to plants and phytoplankton. Among the secreted enzymes, proteases are known to play essential roles in nitrogen cycling in coastal ecosystems. In this study, the protease activities of sediments were assayed at 22 sites in the Kamo River Estuary Tideland. The contour map of the activities showed spots of high activity located in two habitat areas for Uca lactea and Sargassum fulvellum. Moreover, statistical analysis indicated that the protease activity was correlated with the concentrations of organic carbon and nitrogen in the tideland sediments. Actually, loading of sediments with serum albumin as a protein substrate induced marked enhancement of protease activities without increasing of ATP biomass. Electrophoretic analysis clearly revealed the presence of protease bands induced by protein loading. These results suggest that protein loading stimulates the secretion of proteases by certain microorganisms.

在潮滩沉积物中，微生物分泌胞外酶，支持废物净化和为植物和浮游植物补充营养。在分泌的酶中，蛋白酶已知在沿海生态系统的氮循环中发挥重要作用。对卡莫河口潮滩22个站位的沉积物蛋白酶活性进行了测定。活动的等值线图显示，高活动点位于两个栖息地地区的Uca lactea和马尾藻fulvellum。此外，统计分析表明，蛋白酶活性与潮滩沉积物中的有机碳和氮的浓度相关。实际上，负载的沉积物与血清白蛋白作为蛋白质底物诱导蛋白酶活性显着增强，而不增加ATP生物量。电泳分析清楚地揭示了蛋白质加载诱导的蛋白酶条带的存在。这些结果表明，蛋白质负载刺激某些微生物分泌蛋白酶。

项目成果

Regulation of sperm flagellar motility activation and chemotaxis caused by egg-derived substance(s) in sea cucumber sperm

海参精子中卵源性物质对精子鞭毛运动激活和趋化性的调节

• 发表时间: 2009
• 期刊: Cell Motility and the Cytoskeleton Vol.68
• 作者: Morita;M.;M. Kitamura;Nakajima;A. Susilo;E.S.;Takemura;A.;Okuno;M.
• 通讯作者: M.

キウイフルーツに存在する高分子量プロテアーゼの電気泳動による解析

猕猴桃中高分子量蛋白酶的电泳分析

• 发表时间: 2008
• 作者: 増山悦子;中村健一
• 通讯作者: 中村健一

宇宙環境下における生殖・継世代研究の展開-IV宇宙利用シンポジウム報告書

空间环境中繁殖与演替研究进展——第四届空间利用研讨会报告

• 发表时间: 2008
• 期刊: 日本学術会議
• 作者: 奥野誠;中村健一
• 通讯作者: 中村健一

Iron utilizaion and biochemical properties of red tide-causing microalgae WFC2008 5th.

引起赤潮的微藻的铁利用及其生化特性 WFC2008 第 5 期。

• 发表时间: 2008
• 作者: 内藤佳奈子;高瀬真希;今井一郎;中村健一;高橋日出男;Kanako Naito
• 通讯作者: Kanako Naito

Regulation of sperm flagellar motility activation and chemotaxis caused by egg-derived substance(s)in sea cucumber sperm

海参精子卵源性物质对精子鞭毛运动激活和趋化性的调节

• 发表时间: 2009
• 期刊: Cell Motility and the Cytoskeleton 66(4)
• 作者: Morita;M.Okuno;M;et.al
• 通讯作者: et.al