Development of a highly sensitive protein detection method using substrates with nano-scale strcture.

开发使用纳米级结构基质的高灵敏度蛋白质检测方法。

• 批准号: 19550098
• 负责人: YANO Kazuyoshi
• 金额: $ 2.66万
• 依托单位: Tokyo University of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19550098/
• 关键词: プロテオミクス; プラズマ重合; 分析化学; 薄膜; 蛍光

Fluorescence enhancement structure was built up on the glass substrate. First, the Ag film was deposited on the slide glass. The hexamethyldisiloxane plasma-polymerized film (PPF) was subsequently deposited as a dielectric film with various film thickness. The fluorophore-labeled protein was spotted, and the fluorescence intensity from the substrate was measured. As a result, the fluorescence intensity could be enhanced about approximately 13-fold when the thickness of PPF was approximately 56nm, suggesting the future possibility of highly sensitive immunoassay.

在玻璃衬底上建立了荧光增强结构。首先，在载玻片上沉积Ag膜。六甲基二硅氧烷等离子体聚合膜（PPF）随后沉积为具有各种膜厚度的介电膜。点样荧光团标记的蛋白质，并测量来自底物的荧光强度。结果表明，当PPF的厚度约为56nm时，荧光强度可增强约13倍，这表明未来可能实现高灵敏度免疫分析。

项目成果

不一致増幅物質を含むプローブ核酸を用いる標的配列の検出方法、プローブ核酸および標的配列検出用アッセイキット

使用含有错配扩增物质的探针核酸检测靶序列的方法、用于检测探针核酸和靶序列的测定试剂盒

• 发表时间: 2009

プラズマ重合法を用いた抗体アレイの高機能化

使用等离子体聚合改进抗体阵列的功能

• 发表时间: 2009
• 作者: 渡辺直;君塚亮太;山野一行;安田充;池下雄介;宮地寛登;秋元卓央;矢野和義
• 通讯作者: 矢野和義

プラズマ重合法を用いた抗体アレイの高感度化

采用等离子体聚合法的高灵敏度抗体阵列

• 发表时间: 2010
• 作者: 矢野和義;渡辺直;君塚亮太;山野一行;安田充;池下雄介;宮地寛登;秋元卓央
• 通讯作者: 秋元卓央

Fully automated two-dimensional electrophoresis system for high-throughput protein analysis

• DOI: 10.1021/ac070485a
• 发表时间: 2007-08-01
• 期刊: ANALYTICAL CHEMISTRY
• 影响因子: 7.4
• 作者: Hiratsuka, Atsunori;Kinoshita, Hideki;Yokoyama, Kenji
• 通讯作者: Yokoyama, Kenji

A soft and flexible biosensor using a phospholipid polymer for continuous glucose monitoring

• DOI: 10.1007/s10544-009-9300-1
• 发表时间: 2009-08-01
• 期刊: BIOMEDICAL MICRODEVICES
• 影响因子: 2.8
• 作者: Chu, MingXing;Kudo, Hiroyuki;Mitsubayashi, Kohji
• 通讯作者: Mitsubayashi, Kohji