Identification of the gene for a sporeless mutation and its application in edible mushroom breeding.

无孢子突变基因的鉴定及其在食用菌育种中的应用

• 批准号: 19580004
• 负责人: MATSUMOTO Teruyuki
• 金额: $ 2.91万
• 依托单位: Tottori University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19580004/
• 关键词: 食用担子菌類; 胞子欠損性変異; 連鎖解析; AFLPマーカー; 分子育種; ウスヒラタケ

In this study, we constructed the genetic linkage map of the sporeless mutant of Pleurotus pulmonarius based on the DNA markers, and determined the locus of sporeless mutation. Moreover, we obtained the DNA markers which are very tightly linked to this locus. These results enable to carry out the efficient breeding of a sporeless mushrooms. In addition, we cloned the region related to this mutation, and based on the information of linkage map we determined the whole nucleotide sequences of this region. By the comparison of the sequences from mutant and wild type, we found the differences in several gene structures. In future, we will identify the gene that causes the sporeless mutation of this fungus by using the complementation test and so on.

本研究利用DNA标记构建了肺原侧耳无孢子突变体的遗传连锁图谱，并对无孢子突变体的基因位点进行了定位。此外，我们还获得了与该位点紧密连锁的DNA标记。这些结果使得能够进行无孢蘑菇的有效育种。此外，我们还克隆了与该突变相关的区域，并根据连锁图谱信息确定了该区域的全核苷酸序列。通过对突变体和野生型序列的比较，发现了几个基因结构的差异。今后，我们将通过互补试验等方法鉴定引起该真菌无孢子突变的基因。

项目成果

A genetic linkage map of Pleurotus pulmonarius based on AFLP markers, and localization of the gene region for the sporeless mutation

• DOI: 10.1139/g09-021
• 发表时间: 2009-05-01
• 期刊: GENOME
• 影响因子: 3.1
• 作者: Okuda, Yasuhito;Murakami, Shigeyuki;Matsumoto, Teruyuki
• 通讯作者: Matsumoto, Teruyuki

ウスヒラタケ(Pleurotus pulmonarius)における胞子欠損性変異検出STSマーカーの開発

开发用于检测肺侧耳孢子缺陷突变的STS标记

• 发表时间: 2008
• 作者: Inoue Kouichi;Takeshi Tomita;Yoshihara Noriko;Yabuya Tsutomu;奥田康仁・村上重幸・松本晃幸
• 通讯作者: 奥田康仁・村上重幸・松本晃幸

ウスヒラタケ(Pleurotus pulnionarius)における胞子欠損性変異検出STSマーカーの開発

开发用于检测 Pleurotus pulnionarius 孢子缺陷突变的 STS 标记

• 发表时间: 2008
• 作者: 奥田康仁;村上重幸;松本晃幸
• 通讯作者: 松本晃幸

Identification of markers closely linked to the gene region for sporeless mutation in Pleurotus pulmonarius by bulked segregant analysis

通过批量分离分析鉴定与侧耳无孢子突变基因区域密切相关的标记

• 发表时间: 2008
• 作者: Okuda;Y.、Murakami;S.;Matsumoto;T.
• 通讯作者: T.

胞子欠損性変異検出マーカー

孢子缺陷突变检测标记

• 发表时间: 2009