Pure isolation of nitrite-oxidizing bacteria from several environments and development of new phylogenetic marker for them.

从多种环境中纯分离亚硝酸盐氧化细菌并为其开发新的系统发育标记。

• 批准号: 19580096
• 负责人: TAKAHASHI Reiji
• 金额: $ 2.33万
• 依托单位: Nihon University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19580096/
• 关键词: 環境微生物学; 亜硝酸酸化菌; 分子系統分類; 亜硝酸酸化還元酵素遺伝子; nxrB; 新規分離; 分布解析; 微生物群集構造解析; DGGE; Nitrobacter; norB

Nitrite oxidizing bacteria (NOB) that inhabit the whole environment extensively are bacterial species that are in charge of the latter part of nitrification. But little strains of them are isolated as pure culture. This study were carried out for exploration and its application of useful of genetic resources and showed following knowledge. First, as many NOB strains were newly isolated from a variety of environments, we got sufficient strains for studying microbial properties of them. Second, we explored new markers for phylogenetic classification for NOB. Third, we established a new phylogenetic classification with new markers, and we have applied it to "non-culture" analysis of NOB in natural environments.

亚硝酸氧化细菌(NOB)广泛存在于整个环境中，是负责后期硝化作用的细菌。但它们中的一些菌株是作为纯培养分离出来的。本研究对遗传资源的开发利用进行了研究，并取得了以下认识。首先，由于许多NOB菌株是从不同的环境中新分离出来的，所以我们获得了足够的菌株来研究它们的微生物特性。其次，我们探索了新的分子标记用于NOB的系统发育分类。第三，我们用新的标记建立了一个新的系统发育分类，并将其应用于自然环境中NOB的非培养分析。

项目成果

タイ王国酸性硫酸塩土壌から分離されたアンモニア酸化菌のウレアーゼの発現解析

泰国酸性硫酸盐土壤氨氧化细菌脲酶表达分析

• 发表时间: 2008
• 作者: 日比野倫士;高橋令二;中川達功;佐藤一朗;徳山龍明
• 通讯作者: 徳山龍明

Succession and community composition of ammonia-oxidizing archaea and bacteria in bulk soil of a Japanese paddy field

• DOI: 10.1111/j.1747-0765.2010.00449.x
• 发表时间: 2010-04
• 期刊: Soil Science and Plant Nutrition
• 影响因子: 2
• 作者: Chihiro Fujii;T. Nakagawa;Y. Onodera;Naoki Matsutani;Katsuhiro Sasada;R. Takahashi;T. Tokuyama

Characteristics of newly isolated ammonia-oxidizing bacteria from acid sulfate soil and the rhizoplane of leucaena grown in that soil.

酸性硫酸盐土壤中新分离的氨氧化细菌及其生长的银合欢根面的特征。

• 发表时间: 2007
• 期刊: Soil Sci. Plant Nutr 53
• 作者: Satoh;K.;Itoh;C.;Kang;D.-J.;Sumida;H.;Takahashi;R.;Isobe;K.;Sasaki;S.;Tokuyama T
• 通讯作者: Tokuyama T

Seasonal Changes in Abundance of Ammonia-Oxidizing Archaea and Ammonia-Oxidizing Bacteria and Their Nitrification in Sand of an Eelgrass Zone

• DOI: 10.1264/jsme2.me08536
• 发表时间: 2009-01-01
• 期刊: MICROBES AND ENVIRONMENTS
• 影响因子: 2.2
• 作者: Ando, Yoshifumi;Nakagawa, Tatsunori;Tokuyama, Tatsuaki
• 通讯作者: Tokuyama, Tatsuaki

各種環境より分離した亜硝酸酸化菌の特性と新規分類指標の検討

不同环境中亚硝酸氧化菌的特性及新分类指标的检验

• 发表时间: 2007
• 作者: 高橋令二;中川達功;徳山龍明
• 通讯作者: 徳山龍明