Elucidation of fish T lymphocyte subgroup based on the CD4 family marker

基于 CD4 家族标记的鱼类 T 淋巴细胞亚群的阐明

• 批准号: 19580220
• 负责人: J・M Dijkstra
• 金额: $ 2.91万
• 依托单位: Fujita Health University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19580220/
• 关键词: 魚病; 免疫学的予防; CD4; Tリンパ球; 細胞表面マーカー; 水産学; 免疫学; 遺伝子; ゲノム; 生体分子

The main project target was to elucidate the CD4 family of molecules in teleost fish, with clonal ginbuna crucian carp as major fish model. This family of molecules is known to form major markers for leukocyte subpopulations, predominantly of T lymphocytes. We found a homologue of mammalian G6F, a marker of platelets, which we designated G6F-like. We could determine that G6F and G6F-like are related to the CD4 family. A monoclonal antibody against G6F-like was established and we found the molecule expressed in ginbuna thrombocytes and erythrocytes.

本项目主要目的是研究硬骨鱼CD4家族分子，克隆鲫鱼为主要模型鱼。已知这个分子家族形成白细胞亚群的主要标记，主要是T淋巴细胞。我们发现了一种与哺乳动物血小板标记物G6F类似的同源物，我们将其命名为G6F样。我们可以确定G6F和G6F样蛋白与CD4家族有关。建立了抗G6F-like的单克隆抗体，发现该分子在银麻的血小板和红细胞中表达。

项目成果

Evolution of cytokines important for T helper cell differentiation.

对 T 辅助细胞分化很重要的细胞因子的进化。

• 发表时间: 2008
• 作者: Dijkstra JM;Ohtani M;Hayashi N;Nakanishi T;Hashimoto K.
• 通讯作者: Hashimoto K.

Zinc-dependent binding between peptides derived from rainbow trout CD8a and LCK.

虹鳟鱼 CD8a 和 LCK 衍生肽之间的锌依赖性结合。

• 发表时间: 2010
• 期刊: Fish Shellfish Immunology 28
• 作者: Hayashi N;Takeuchi M;Nakanishi T;Hashimoto K;Dijkstra JM.
• 通讯作者: Dijkstra JM.

A molecule in teleost fish, related with human MHC-encoded G6F, has a cytoplasmic tail with ITAM and marks the surface of thrombocytes and in some fishes also of erythrocytes

• DOI: 10.1007/s00251-010-0460-1
• 发表时间: 2010-08-01
• 期刊: IMMUNOGENETICS
• 影响因子: 3.2
• 作者: Ohashi, Ken;Takizawa, Fumio;Dijkstra, Johannes Martinus
• 通讯作者: Dijkstra, Johannes Martinus

Zinc-dependent binding between peptides derived from rainbow trout CD8alpha and LCK

虹鳟鱼 CD8α 和 LCK 衍生肽之间的锌依赖性结合

• 发表时间: 2010
• 期刊: Fish Shellfish Immunol 28
• 作者: Hayashi N;Takeuchi M;Nakani shi T;Hashi moto K;Djkstra JM.;Hayashi N
• 通讯作者: Hayashi N

Molecular characteristics of the leukocyte markers CD4-1, CD4-2, LAG-3, CD8alpha and CD8beta in teleost fish.

硬骨鱼白细胞标记 CD4-1、CD4-2、LAG-3、CD8α 和 CD8β 的分子特征。

• 发表时间: 2009
• 作者: Dijkstra JM;Hayashi N;Nakanishi T;Hashimoto K.
• 通讯作者: Hashimoto K.