Mature Adipocyte-Derived Dedifferentiated Fat Cells Can Transdifferentiate Into Neural Cells And Promote Functional Recovery From Spinal Cord Injury-Induced Motor Dysfunction In Rodents

成熟脂肪细胞衍生的去分化脂肪细胞可以转分化为神经细胞并促进啮齿类动物脊髓损伤引起的运动功能障碍的功能恢复

• 批准号: 19580348
• 负责人: KANO Koichiro
• 金额: $ 3万
• 依托单位: Nihon University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19580348/
• 关键词: 脂肪細胞; 脱分化; 分化転換; 神経系細胞

When mature adipocytes were isolated from adipose tissue and grown in ceiling culture, transformation into fibroblast-like cells without lipid droplets occurred. These fibroblast-like cells, termed de-differentiated fat (DFAT) cells, could proliferate and could also redifferentiate into mature adipocytes. DFAT cells expressed neural markers such as nestin, NSE, βIII tubulin, NF, S100, GFAP and CNPase. Allografting of DFAT cells into spinal cord injury (SCI)-induced rodents led to significant recovery from hindlimb dysfunction. Grafted cells were detected at the injection site, and some of these cells expressed βIII tubulin. These results indicate that DFAT cells can transdifferentiate into neural cells, and contribute to the promotion of functional recovery. These findings also suggest that mature adipocytes could become a new source for cell replacement therapy to treat central nervous system disorders.

当成熟脂肪细胞从脂肪组织中分离并在天花板培养中生长时，发生转化为成纤维细胞样细胞而没有脂滴。这些成纤维细胞样细胞，称为去分化脂肪（DFAT）细胞，可以增殖，也可以再分化为成熟的脂肪细胞。DFAT细胞表达nestin、NSE、βIII微管蛋白、NF、S100、GFAP和CNB等神经标志物。DFAT细胞移植到脊髓损伤（SCI）诱导的啮齿动物导致后肢功能障碍的显着恢复。在注射部位检测到移植细胞，其中一些细胞表达βIII微管蛋白。这些结果表明，DFAT细胞可以转分化为神经细胞，并有助于促进功能恢复。这些发现还表明，成熟脂肪细胞可能成为细胞替代疗法治疗中枢神经系统疾病的新来源。

项目成果

Establishment and Characteristics of Porcine Preadipocyte Cell Lines Derived From Mature Adipocytes

• DOI: 10.1002/jcb.22431
• 发表时间: 2010-02-15
• 期刊: JOURNAL OF CELLULAR BIOCHEMISTRY
• 影响因子: 4
• 作者: Nobusue, Hiroyuki;Kano, Koichiro
• 通讯作者: Kano, Koichiro

前駆脂肪細胞の分化程度は移植後の脂肪組織形成に影響を及ぼす

前脂肪细胞分化程度影响移植后脂肪组织的形成

• 发表时间: 2008
• 作者: 信末博行;飯島明日香;遠藤克;加野浩一郎
• 通讯作者: 加野浩一郎

Mature Adipocyte-Derived Dedifferentiated Fat Cells Can Trans-Differentiate into Osteoblasts In Vitro and In Vivo only by All-Trans Retinoic Acid

• DOI: 10.1247/csf.08038
• 发表时间: 2008-01-01
• 期刊: CELL STRUCTURE AND FUNCTION
• 影响因子: 1.5
• 作者: Oki, Yoshinao;Watanabe, Saiko;Kano, Koichiro
• 通讯作者: Kano, Koichiro

成熟脂肪細胞に由来する多能性前駆細胞DFATの樹立と特性

成熟脂肪细胞来源的多能祖细胞 DFAT 的建立和特性

• 发表时间: 2009
• 作者: Matsumoto T;Kano K;et. al.;加野浩一郎
• 通讯作者: 加野浩一郎

Establishment of a preadipocyte cell line derived from mature adipocytes of GFP transgenic mice and formation of adipose tissue

• DOI: 10.1007/s00441-008-0593-9
• 发表时间: 2008-06-01
• 期刊: CELL AND TISSUE RESEARCH
• 影响因子: 3.6
• 作者: Nobusue, Hiroyuki;Endo, Tsuyoshi;Kano, Koichiro
• 通讯作者: Kano, Koichiro