由于对植物细胞“全能性”的调控机理认识不足，目前大多数农作物缺乏高频再生体系，严重制约了重要功能基因在农作物改良中的应用。本项目围绕“启动植物体细胞脱分化的充分和必要条件”这一科学问题，在前期鉴定得到“类根愈伤组织”起始关键促进子ARF10及其互作蛋白HBI1的基础上，拟开展以下研究：（1）分析ARF10与HBI1对类根愈伤组织起始的调控作用、二者的共定位关系及遗传学关系，明确HBI1与ARF10共定位信号是否可以作为“脱分化”起始的标志组分；（2）分析ARF10与HBI1如何协同调控共同靶基因WOX12的表达，确定三者的共定位关系及遗传学关系，明确HBI1/ARF10/WOX12共定位信号是否可以作为“脱分化”起始的标志组分；（3）利用RNA-seq和ChIP-seq技术，获得HBI1与ARF10协同调控的基因网络。相关成果可为农作物高频再生体系的建立提供理论指导。

Because of the poor understanding of the mechanisms underlying the totipotent of plant cells, few efficient plant regeneration systems of crops have been established until now, which severely restricts the application of functional genes in crop improvement. A key promoter of callus initiation ARF10 and its interacting protein HBI1 were identified in our previous study. In order to explore the sufficient and necessary conditions of callus initiation, the following experiments will be carried out in this study: (1) analyze the function of HBI1 and ARF10 in callus initiation and the co-localization and genetic relationship of them; (2) analyze the regulation of WOX12 by HBI1 and ARF10 during callus initiation and the co-localization and genetic relationship of HBI1, ARF10 and WOX12; (3) analyze the gene network downstream of HBI1 and ARF10. The results will shed lights on the mechanism of plant cell totipotent and make the establishment of efficient crop regeneration systems easier.