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Ultrafast determination of drugs in biological samples by column-switching LC/MS

通过柱切换 LC/MS 超快速测定生物样品中的药物

基本信息

批准号：
19590683
负责人：
ARINOBU Tetsuya
金额：
$ 2.91万
依托单位：
Aichi Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2007
资助国家：
日本
起止时间：
2007 至 2009
项目状态：
已结题

项目摘要

Theophylline and caffeine are natural alkaloids widely being ingested, and show various pharmacological effects in humans. Caffeine is a constituent of coffee and other beverages and included in many medicines. Theophylline is used as a bronchodilator and also formed as a metabolite of caffeine in humans ; theophylline poisoning is occasionally encountered in the field of forensic toxicology, because of its relatively narrow safe range of therapeutic doses. They have been analyzed by various methods, such as gas chromatography (GC), GC-mass spectrometry (MS), high-performance (HP) liquid chromatography (LC)-MS. In the existing analytical methods for the drugs, the sample pretreatments such as liquid-liquid extraction, solid-phase extraction, filtration or centrifugation were usually required to remove proteins and other impurities contained in biological matrices. The analytical methods including time-consuming multi-step sample pretreatments are unsuitable for rapid detection of poisonous materials in human specimens. To avoid such sample pretreatments, special HPLC columns, which enabled direct injection of crude biological samples, were developed ; our group applied them to compounds of forensic toxicological interest. Furthermore, backflush column-switching system using one of special HPLC columns have become a powerful analytical tool for simultaneous determinations of various drugs in biological samples. In this study, we have established an automated on-line method for ultra-fast determination of theophylline and caffeine in human serum by HPLC-MS with backflush column-switching and a monolithic separation column. To our knowledge, our method presented in this study is the fastest MS determination technique for theophylline and caffeine in human serum using the conventional HPLC instrument.

茶碱和咖啡因是广泛摄入的天然生物碱，在人体内表现出多种药理作用。咖啡因是咖啡和其他饮料的一种成分，并包含在许多药物中。茶碱被用作支气管扩张剂，也作为咖啡因在人体内的代谢物形成;茶碱中毒偶尔会在法医毒理学领域遇到，因为其相对较窄的安全治疗剂量范围。在现有的药物分析方法中，通常需要对样品进行液-液萃取、固相萃取、过滤或离心等预处理，以去除生物基质中的蛋白质等杂质。分析方法包括耗时的多步骤样品前处理，不适合人体标本中有毒物质的快速检测。为了避免这样的样品预处理，特殊的HPLC柱，使直接注射的粗生物样品，被开发出来;我们的小组将它们应用于法医毒理学感兴趣的化合物。此外，反冲洗柱切换系统使用一个特殊的高效液相色谱柱已成为一个强大的分析工具，同时测定生物样品中的各种药物。本文建立了一种反冲洗柱切换和整体柱分离的高效液相色谱-质谱联用仪在线自动化超快速测定人血清中茶碱和咖啡因的方法。据我们所知，我们在这项研究中提出的方法是最快的质谱测定技术，茶碱和咖啡因在人血清中使用传统的高效液相色谱仪。

项目成果

期刊论文数量（0）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

High-throughput determination of theophylline and caffeine in human serum by conventional liquid chromatography-mass spectrometry

DOI：
10.1007/s11419-008-0058-6
发表时间：
2009-02-01
期刊：
FORENSIC TOXICOLOGY
影响因子：
2.2
作者：
Arinobu, Tetsuya;Hattori, Hideki;Seno, Hiroshi
通讯作者：
Seno, Hiroshi

Determination of phenothiazines in human serum by HPLC/SSI-MS using backflush column-switching

采用反吹柱切换的 HPLC/SSI-MS 测定人血清中的吩噻嗪

DOI：
发表时间：
2007
期刊：
影响因子：
0
作者：
Arinobu T.;Aoki R.;Kumazawa T.;Lee X.-P.;Ishii A.;Kaneko R.;Kojima S.;Noguchi H.;Suzuki O.;Hattori H.;Seno H.
通讯作者：
Seno H.

Rapid analysis of sertraline, fluvoxamine, and paroxetine in serum specimens by LC-MS-MS using a new polymer column

使用新型聚合物色谱柱通过 LC-MS-MS 快速分析血清样本中的舍曲林、氟伏沙明和帕罗西汀

DOI：
发表时间：
2007
期刊：
Forensic Toxicology 25
影响因子：
0
作者：
Hideki Hattori;Kenjiro Ito;Masae Iwai;Tetsuya Arinobu;Yoko Mizutani;Takeshi Kumazawa;Akira Ishii;Osamu Suzuki;Hiroshi Seno
通讯作者：
Hiroshi Seno

An automated on-line method for simultaneous analysis of phenothiazines in human serum by high-performance liquid chromatography/sonic spray ionization mass spectrometry using backflush column switching.

一种使用反吹柱切换的高效液相色谱/声波喷雾电离质谱法同时分析人血清中吩噻嗪的自动化在线方法。