Relationship between polymorphisms of eNOS gene and depression

eNOS基因多态性与抑郁症的关系

基本信息

批准号：
19591385
负责人：
YOSHIMURA Reiji
金额：
$ 2.5万
依托单位：
University of Occupational and Environmental Health, Japan
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2007
资助国家：
日本
起止时间：
2007 至 2009
项目状态：
已结题

项目摘要

Objective : We hypothesized that polymorphisms of eNOS gene are associated with pathogenesis of depression. To confirm the hypothesis, we examined the association of the polymorphisms of eNOS gene between patients with MDD. In addition, we also investigated the associations among the polymorphisms of eNOS gene among scores of HAMD and Beck Depression Inventory (BDI), ankle brachial index (ABI) and plasma NOx levels in depressed patients.Materials and Methods : This study included 103 in- or outpatients in our university hospital who met the DSM-IV-TR criteria for MDD (M/F:41/62, age:44±13 years) and 104 age- and sex-matched healthy controls (M/F:37/66, age:39±16 years). Based on three polymorphism of eNOS gene (SNP; rs2070744, rs1799983, VNTR in intron 4), we conducted a genetic association analysis of case- control samples in patients with MDD or healthy controls in Japanese population. The plasma NOx levels were analyzed by the Griess method, using ENO-20. We used TaqMan assay for two SNPs, and the VNTR polymorphisms in intron 4 were determined by PCR-RFLP.Results : We did not find genotype distributions between patients with MDD and healthy volunteers. No associations were observed between any polymorphisms of eNOS gene and HAMD, BDI, or ABI in patients with MDD. Conclusions : These results suggest that the three polymorphisms of eNOS gene might play little roles for the pathogenesis of depression.

目的：我们假设eNOS基因的多态性与抑郁发病机理有关。为了证实该假设，我们检查了MDD患者之间eNOS基因的多态性的关联。 In addition, we also investigated the associations among the polymorphisms of eNOS gene among scores of HAMD and Beck Depression Inventory (BDI), ankle brachial index (ABI) and plasma NOx levels in depressed patients.Materials and Methods: This study included 103 in- or outpatients in our university hospital who met the DSM-IV-TR criteria for MDD (M/F:41/62,年龄：44±13岁）和104岁和性别匹配的健康对照（M/F：37/66，年龄：39±16岁）。基于ENOS基因的三种多态性（SNP； RS2070744，RS1799983，内含子4中的VNTR），我们对日本人群中MDD或健康对照患者的病例对照样本进行了遗传关联分析。使用ENO-20通过GRIESS方法分析血浆NOX水平。我们使用了两个SNP的Taqman评估，并且内含子4中的VNTR多态性由PCR-RFLP确定：我们没有发现MDD和健康志愿者患者之间的基因型分布。 MDD患者的ENOS基因和HAMD，BDI或ABI的任何多态性之间均未观察到任何关联。结论：这些结果表明，eNOS基因的三种多态性可能对抑郁的发病机理起很少作用。