Effects of miRNA alterations on the chemosensitivity against anticancer agents in gastrointestinal tract tumors

miRNA改变对胃肠道肿瘤抗癌药物化疗敏感性的影响

• 批准号: 19591558
• 负责人: IKEDA Kenichiro
• 金额: $ 2.75万
• 依托单位: Iwate Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19591558/
• 关键词: 消化器癌; 化学療法; REST; TUBB3; miRNA; microRNA; がん; がん遺伝子; がん抑制遺伝子; 薬剤耐性; ABCB1; epigenetics; CDDP; 5-FU; Paclitaxel

To investigate the relationship of miRNA alterations and chemosensitity, we investigated hsa-miR-451 and hsa-miR-498 expression in gastrointestinal tract tumors. Further, we examined also methylation and acetylation statues of TUBB3 promoter region. Demethylation and hyperacetylation at the promoter well correlated with overexpression of TBBU3 proteinn expression. Down-regulation of hsa-miR-451 and hsa-miR-498 induced ABCG2 overexpression. These results suggested that alterations of miRNA expression may contribute to chemosensitivity for anticancer drugs.

为了研究miRNA改变与化疗敏感性的关系，我们研究了hsa-miR-451和hsa-miR-498在胃肠道肿瘤中的表达。此外，我们还检查了TUBB 3启动子区的甲基化和乙酰化状态。启动子的去甲基化和高乙酰化与TBBU 3蛋白的过度表达密切相关。hsa-miR-451和hsa-miR-498的下调诱导ABCG 2过表达。这些结果表明，miRNA表达的改变可能有助于抗癌药物的化疗敏感性。

项目成果

Endoscopic thyroidectomy by the breast approach: A single institution's 9-year experience

• DOI: 10.1007/s00268-007-9375-x
• 发表时间: 2008-03-01
• 期刊: WORLD JOURNAL OF SURGERY
• 影响因子: 2.6
• 作者: Sasaki, Akira;Nakajima, Jun;Wakabayashi, Go
• 通讯作者: Wakabayashi, Go

Detection of human papillomavirus in a urothelial carcinoma mimicking urethral caruncle.

在模拟尿道肉阜的尿路上皮癌中检测人乳头瘤病毒。

• 发表时间: 2010
• 期刊: Int J Urol 17
• 作者: Y. Tatemichi;H. Oikawa;C. Maesawa;J. Ambo;M. Sato;H. Koike;T. Sata;T. Fujioka;T. Masuda
• 通讯作者: T. Masuda

Chitin/cisplatin-an adhesive anticancer drug

甲壳素/顺铂——一种粘附性抗癌药

• 发表时间: 2008
• 期刊: Gan To Kagaku Ryoho 35
• 作者: Sugitachi A;Otsuka K;Itabashi T;Chiba T;Kimura Y;Sasaki A;Ikeda K;Uesugi N;Takamori Y;Kurozumi S;Mori T;Wakabayashi G.
• 通讯作者: Wakabayashi G.

Progress in allergy signal research on mast cells : the role of histamine in goblet cell hyperplasia in allergic airway inflammation-a study using the Hdc knockout mouse.

肥大细胞过敏信号研究进展：组胺在过敏性气道炎症杯状细胞增生中的作用——利用Hdc基因敲除小鼠的研究。

• 发表时间: 2008
• 期刊: Journal of Pharmacological Science 106
• 作者: Yamauchi K;Piao HM;Nakadate T;Shikanai T;Nakamura Y;et al.
• 通讯作者: et al.

Epigenetic modification is involved in aberrant expression of class III beta-tubulin, TUBB3, in ovarian cancer cells.

表观遗传修饰与卵巢癌细胞中 III 类 β-微管蛋白 TUBB3 的异常表达有关。

• 发表时间: 2008
• 期刊: Int J Oncol 32
• 作者: Izutsu N;Maesawa C;Masuda T.;et al
• 通讯作者: et al