Treatment for primary lung graft dysfunction by transfusion of endothelial progenitor cells transferred with intreleukin-10 gene.

通过输注转有白介素 10 基因的内皮祖细胞治疗原发性肺移植功能障碍。

• 批准号: 19591608
• 负责人: OKADA Yoshinori
• 金额: $ 2.83万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19591608/
• 关键词: 肺移植; 血管内皮前駆細胞; インターロイキン-10; GFPラット; 虚血・再灌流傷害; 細胞移植; 血管内皮前前駆細胞; IL-10; 組胞移植

The purpose of this research project was to examine whether regeneration of pulmonary vascular endothelial cells that are derived from recipient cells can be observed in the rat lung in a rat model of lung transplantation, and whether transplantation of endothelial progenitor cells derived from bone marrow can facilitate regeneration of pulmonary endothelial cells. We performed rat lung transplantation from the SD rat donor to the GFPtg SD rat recipient and observed GFP(+), CD31(+) cell in the lung isograft, suggesting regeneration of endothelial cells derived from recipient cells occurs. We are planning recipient bone marrow cell transplantation to examine whether it can facilitate regeneration of pulmonary endothelial cells and also reduce ischemia-reperfusion injury of the lung isograft.

本课题的研究目的是在大鼠肺移植模型中，观察是否可以观察到来自受体细胞的肺血管内皮细胞在大鼠肺中再生，以及移植来自骨髓的内皮祖细胞是否可以促进肺内皮细胞的再生。我们将SD大鼠供体的大鼠肺移植到GFPtg SD大鼠受体中，观察到肺同种移植物中的GFP（+）、CD31（+）细胞，表明受体细胞衍生的内皮细胞发生再生。我们正在计划受体骨髓细胞移植，以观察是否能促进肺内皮细胞的再生，并减轻肺同种移植物的缺血-再灌注损伤。

项目成果

Preservation Solution for Endogenous Tisssue Stem Cells

内源性组织干细胞保存液

• 发表时间: 2009
• 作者: Suzuki T;Kubo H;Fujino N;Hegab AE;He M;Okada Y;Yamaya M;Kondo T.
• 通讯作者: Kondo T.

CD117 Positive Cells without Mast Cell Lineage Are Localized in the Lung Parenchyma and Alveolar Wall in Adult Human Lungs

无肥大细胞谱系的 CD117 阳性细胞位于成人肺的肺实质和肺泡壁中

• 发表时间: 2009
• 期刊: Am J Respir Crit Care Med 179
• 作者: Suzuki T;et al.
• 通讯作者: et al.

Preservation Solution for Endogenous Tisssue Stem Cells.

内源组织干细胞保存液。

• 发表时间: 2009
• 期刊: Am J Respir Crit Care Med 179
• 作者: T.Suzuki;H.Kubo;N.Fujino;A.E.Hegab;M.He;Y.Okada;M.Yamaya;T.Kondo.
• 通讯作者: T.Kondo.

Transbronchial human interleukin-10 gene transfer reduces acute inflammation associated with allograft rejection and intragraft interleukin-2 and tumor necrosis factor-α gene expression in a rat model of lung transplantation

• DOI: 10.1016/j.healun.2009.10.002
• 发表时间: 2010-03-01
• 期刊: JOURNAL OF HEART AND LUNG TRANSPLANTATION
• 影响因子: 8.9
• 作者: Oishi, Hisashi;Okada, Yoshinori;Kondo, Takashi
• 通讯作者: Kondo, Takashi