Systemic Gene Therapy for Osteosarcoma by Tumor Suppressor Gene Fus1

抑癌基因Fus1对骨肉瘤的全身基因治疗

• 批准号: 19791021
• 负责人: KAWASHIMA Hiroyuki
• 金额: $ 2.01万
• 依托单位: Niigata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2008
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19791021/
• 关键词: FUS1; 間葉系腫瘍; RT-PCR; Western blot; Tissue Micro Array; 骨軟部腫瘍; real time RT-PCR

本研究では、間葉系正常細胞、組織と間葉系腫瘍細胞、組織における癌抑制遺伝子FUS1の発現を検討した。FUS1のRNAは正常、腫瘍の両組織、細胞で広く発現が認められたが、蛋白は正常の末梢神経と正常線維芽細胞とごく一部の骨肉腫などの腫瘍組織にのみ発現が認められた。FUS1遺伝子導入による間葉系悪性腫瘍細胞の増殖抑制とアポトーシスの誘導については、骨肉腫など多くの細胞株に効果を認めたが、程度は様々であった。肺癌細胞を用いたヌードマウスの同所移植モデルにおいて、FUS1静脈注入法による遺伝子治療の効果が確認できた。

This study investigated the development of tumor suppressor gene FUS1 in normal and mesenchymal cells and tissues. FUS1 RNA is normal, tumor tissue, cell development is recognized, protein is normal, peripheral nerve is normal, cell development is recognized, part of tumor tissue development is recognized. FUS1 gene introduction into mesenchymal tumor cells, inhibition of proliferation and induction of tumor cells Lung cancer cells were transplanted with FUS1, and the results were confirmed.

项目成果

Nanoparticla-mediated delivery of therapeutic peptide derived from novel tumor suppressor FUS1 protein for human lung cancer treatment

纳米颗粒介导的源自新型肿瘤抑制 FUS1 蛋白的治疗肽的递送用于治疗人肺癌

• 发表时间: 2007
• 作者: Deng W;Uno F;Wu G;Kawashima H;et al.
• 通讯作者: et al.

Overcoming gefitinib/erlotinib-induced resistance by tumor suppressor fus1-madiated downregulation of met/akt signaling in human lung cancer

通过肿瘤抑制因子fus1介导的met/akt信号下调克服吉非替尼/厄洛替尼诱导的耐药性

• 发表时间: 2008
• 作者: Deng W;Kawashima H;et al.
• 通讯作者: et al.

Synergistic tumor suppression by coexpression of FUS1 and p53 is associated with down-regulation of murine double minute-2 and activation of the apoptotic protease-activating factor 1-dependent apoptotic pathway in human non-small cell lung cancer cells

• DOI: 10.1158/0008-5472.can-06-3463
• 发表时间: 2007-01-15
• 期刊: CANCER RESEARCH
• 影响因子: 11.2
• 作者: Deng, Wu-Guo;Kawashima, Hiroyaki;Ji, Lin
• 通讯作者: Ji, Lin

Interaction of tumor suppressor FUS1 with nuclear receptor tyrosine kinase RAR-alpha proteins enhances the sensitivity of non-small cell lung cancer (NSCLC) to retinoid-mediated therapy

肿瘤抑制因子 FUS1 与核受体酪氨酸激酶 RAR-α 蛋白的相互作用增强非小细胞肺癌 (NSCLC) 对类维生素A介导的治疗的敏感性

• 发表时间: 2007
• 作者: Iwamaru A;Deng W;Kawashima H;et al.
• 通讯作者: et al.