Regeneration of Enamel using Cultured Ameloblast

使用培养的成釉细胞再生牙釉质

• 批准号: 19791448
• 负责人: SHINOHARA Yoshinori
• 金额: $ 2.42万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19791448/
• 关键词: 歯科用材料; 歯科理工学; 歯学; 再生医学; 細胞・組織

The fundamental treatment for a tooth defect is to regenerate those teeth that have been lost as a result of the defect. However, in order to apply this tooth organ regeneration to medical treatment, issues such as tooth eruption, control of size and morphology, regeneration period, are left as pitfalls. Thus, this is not regarded as a foolproof treatment. Therefore, at present prosthesis treatments such as bridge, partial denture and dental implant are commonly performed. On the other hand, trauma or congenital/acquired tooth defect is restored by artificial materials, such as metal, ceramic and resin. However, it is true that these artificial materials have various issues in terms of biocompatibility, esthetics, functionality, compared to natural teeth. This study delineates how regenerative therapy is applied to clinical application. As a first step, we examined tooth regeneration which applied tissue engineering technique, especially enamel regeneration and tooth prosthesis material. Recently, enamel regeneration, which utilizes culture tooth bud cell, has been reported. However, it is found that not only does enamel regeneration take a long period to complete tissue regeneration but also turn regenerated tissue premature and small. In addition, it has been reported that the extracellular matrix including collagen type 1 and fibronectin promoted the growth and differentiation of cultured dental epithelial cell. This research examines how cultured odintogenic cell stratification will affect the ability of cell growth and differentiation and tissue regeneration. Then, it intends to prove cell culture and tissue regeneration method quantitatively and qualitatively.

牙齿缺损的基本治疗方法是再生那些因缺损而失去的牙齿。然而，为了将这种牙齿器官再生应用于医学治疗，诸如牙齿萌出、大小和形态的控制、再生期等问题成为陷阱。因此，这并不被认为是一种万无一失的治疗方法。因此，目前修复治疗，如桥梁，部分义齿和牙科植入物通常进行。另一方面，创伤或先天性/后天性牙齿缺损通过人工材料，如金属、陶瓷和树脂修复。然而，与天然牙齿相比，这些人造材料在生物相容性、美学、功能性方面确实存在各种问题。这项研究描述了再生疗法如何应用于临床应用。本文首先对应用组织工程技术的牙再生，特别是牙釉质再生和牙修复材料进行了研究。近年来，利用培养的牙胚细胞进行牙釉质再生的研究已有报道。然而，人们发现，釉质再生不仅需要很长的时间才能完成组织再生，而且会使再生组织过早变小。此外，有报道称细胞外基质包括1型胶原和纤维连接蛋白促进培养的牙上皮细胞的生长和分化。本研究探讨了成牙本质细胞分层对细胞生长、分化和组织再生能力的影响。然后，对细胞培养和组织再生方法进行定量和定性的验证。