Analysis of the protein-turnover mechanisms in the chloroplast

叶绿体中蛋白质周转机制的分析

• 批准号: 19687003
• 负责人: TANAKA Ryouichi
• 金额: $ 7.74万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (A)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19687003/
• 关键词: 葉緑体; プロテアーゼ; シロイヌナズナ

We found that a specific sequence in a chloroplast protein, chlorophyllide a oxygenase, is essential in the turnover of this protein. This sequence is most likely a degron for the chloroplast Clp protease. We obtained evidence for the presence of similar degrons in the other chloroplast proteins, indicating that the protein turnover mechanism is rather general in the chloroplast.

我们发现叶绿体蛋白（叶绿素加氧酶）中的特定序列对该蛋白的周转至关重要。该序列很可能是叶绿体 Clp 蛋白酶的降解决定子。我们获得了其他叶绿体蛋白质中存在类似降解决定子的证据，表明蛋白质周转机制在叶绿体中相当普遍。

项目成果

Loss of the N-terminal domain of chlorophyllide a oxygenase induces photodamage during greening of Arabidopsis seedlings.

• DOI: 10.1186/1471-2229-8-64
• 发表时间: 2008-06-12
• 期刊: BMC plant biology
• 影响因子: 5.3
• 作者: Yamasato A;Tanaka R;Tanaka A
• 通讯作者: Tanaka A

Identification of a novel vinyl reductase gene essential for the biosynthesis of monovinyl chlorophyll in Synechocystis sp.

集胞藻中单乙烯基叶绿素生物合成所必需的新型乙烯基还原酶基因的鉴定。

• 发表时间: 2008
• 期刊: PCC6803 J Biol Chem 283
• 作者: Ito H;Yokono M;Tanaka R Tanaka A
• 通讯作者: Tanaka R Tanaka A

Participation of Chlorophyll b Reductase in the Initial Step of the Degradation of Light-harvesting Chlorophyll a/b-Protein Complexes in Arabidopsis

• DOI: 10.1074/jbc.m109.008912
• 发表时间: 2009-06-26
• 期刊: JOURNAL OF BIOLOGICAL CHEMISTRY
• 影响因子: 4.8
• 作者: Horie, Yukiko;Ito, Hisashi;Tanaka, Ayumi
• 通讯作者: Tanaka, Ayumi

Deregulated Chlorophyll b Synthesis Reduces the Energy Transfer Rate Between Photosynthetic Pigments and Induces Photodamage in Arabidopsis thaliana

• DOI: 10.1093/pcp/pcq050
• 发表时间: 2010-06-01
• 期刊: PLANT AND CELL PHYSIOLOGY
• 影响因子: 4.9
• 作者: Sakuraba, Yasuhito;Yokono, Makio;Tanaka, Ayumi
• 通讯作者: Tanaka, Ayumi

Identification of a gene essential for protoporphyrinogen IX oxidase activity in the cyanobacterium Synechocystis sp. PCC6803

• DOI: 10.1073/pnas.1000771107
• 发表时间: 2010-09-21
• 期刊: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• 影响因子: 11.1
• 作者: Kato, Kazushige;Tanaka, Ryouichi;Hosaka, Hideo
• 通讯作者: Hosaka, Hideo