Towards a comprehensive understanding of the chlorophyll breakdown pathway

全面了解叶绿素分解途径

• 批准号: 23570042
• 负责人: TANAKA Ryouichi
• 金额: $ 3.49万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23570042/
• 关键词: クロロフィル

For better understanding of the chlorophyll breakdown pathway in plants, we investigated the function of chlorophyllase, a common plant enzyme that catalyzes the cleavage of chlorophyll to form chlorophyllide and phytol. We found that this enzyme is localized in the endoplasmic reticulum and the tonoplast. This enzyme actively catalyzes chlorophyllide formation only when cells are disrupted. Arabidopsis leaves with genetically enhanced CLH activity exhibit toxicity when fed to Spodoptera larvae, an insect herbivore. Purified chlorophyllide partially suppresses the growth of the larvae. We showed that chlorophyllase and chlorophyll form a two-component defense system against chewing herbivores.

为了更好地理解植物中叶绿素的分解途径，我们研究了叶绿素酶的功能，叶绿素酶是一种常见的植物酶，催化叶绿素分解形成叶绿素和叶绿醇。 我们发现这种酶定位于内质网和液泡膜。这种酶只有在细胞被破坏时才能积极催化叶绿素的形成。拟南芥叶片与遗传增强CLH活性表现出毒性时，饲料的夜蛾幼虫，一种昆虫食草动物。纯化叶绿素部分抑制幼虫的生长。我们发现，叶绿素酶和叶绿素形成一个双组分防御系统对咀嚼食草动物。

项目成果

Possibleinvolvement of chlorophyllase in a plantdefense system

叶绿素酶可能参与植物防御系统

• 发表时间: 2013
• 作者: Xueyun Hu;Masanori Oshiai;TohruTsuchiya;Stefan Hortensteiner;Ayumi Tanaka;Ryouichi Tanaka
• 通讯作者: Ryouichi Tanaka

Chlorophyll metabolism in photosynthetic organisms.

光合生物中的叶绿素代谢。

• DOI: 10.1142/9789814335508_fmatter05
• 发表时间: 2012
• 期刊: Handbook of Porphyrin Science
• 作者: Tanaka R;Takabayashi A;Ito H and Tanaka A
• 通讯作者: Ito H and Tanaka A

Chlorophyllase 1 is not involved in chlorophyll breakdown during methyl-jasmonate-induced programmed cell death in Arabidopsis thaliana

在拟南芥茉莉酸甲酯诱导的程序性细胞死亡过程中，叶绿素酶 1 不参与叶绿素分解

• 发表时间: 2012
• 作者: Xueyun Hu;Tohru Tsuchiya;Stefan Hortensteiner;Ayumi Tanaka and Ryouichi Tanaka
• 通讯作者: Ayumi Tanaka and Ryouichi Tanaka

Chlorophyllase1 is not involved in chlorophyll breakdown during methyl-jasmonate-induced senescence in Arabidopsis thaliana.

叶绿素酶 1 不参与茉莉酸甲酯诱导拟南芥衰老过程中的叶绿素分解。

• 发表时间: 2012
• 作者: Xueyun Hu;Tohru Tsuchiya;Stefan Hörtensteiner;Ayumi Tanaka;Ryouichi Tanaka
• 通讯作者: Ryouichi Tanaka

Simple extraction methods that prevent the artifactual conversion of chlorophyll to chlorophyllide during pigment isolation from leaf samples.

• DOI: 10.1186/1746-4811-9-19
• 发表时间: 2013-06-19
• 期刊: Plant methods
• 影响因子: 5.1
• 作者: Hu X;Tanaka A;Tanaka R
• 通讯作者: Tanaka R