Geobiology of saline fluids from the deep biosphere obtained from the KTB pump test

KTB 泵测试获得的深层生物圈含盐流体的地球生物学

• 批准号: 5364122
• 负责人: Professor Dr. Joachim Reitner
• 金额: --
• 依托单位: Abteilung Geobiologie
• 依托单位国家: 德国
• 项目类别: Infrastructure Priority Programmes
• 财政年份: 2002
• 资助国家: 德国
• 起止时间: 2001-12-31 至 2005-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5364122?language=en
• 关键词: Geobiology; saline; fluids; deep; biosphere

The major goal of the proposed project is the characterization of microbial biofilms formed in controlled environment biofilm model systems inoculated and maintained with fluids from the KTB pumping test originated from the deep continental biosphere. For this purpose, two controlled environment biofilm model systems are designated for the investigation of biofilm formation. System A is based on a modified Robbins Devices (MRD), that allows testing of different substrata, and coupons can be removed independently after various exposure times for examination of the biofilm or testing the effects of different media on biofilm growth in situ. Thin sections of different types of rock will be used as natural substrata for biofilm formation. For biofilm formation of putative thermophilic Archaea and Bacteria obtained from the deep subsurface fluids, a modified heatable chemostat (System B) is designated. The biofilms obtained from both systems will be investigated with regard to their overall phylogenetic structure, spatial arrangement and the cellular activity of the biofilm associated bacteria using fluorochromes for total cell count determination and fluorescent in situ hybridization (FISH). Analysis of the signature lipid biomarkers additonally provides quantitative measures of abundance, distribution and the nutritional status of the attached communities. Along with the microbiological investigations, biochemical analysis, especially for d13C, d15N, 2H isotopic compositions of specific organic compounds in the fluids (e.g. lipids) will be performed that could enable to elucidate microbiological driven processes within the deep biosphere.

拟议项目的主要目标是在受控环境生物膜模型系统中形成的微生物生物膜的表征，该模型系统接种并维持来自深层大陆生物圈的KTB泵送试验的流体。为此，两个受控环境生物膜模型系统被指定为生物膜形成的调查。系统A基于改良的Robbins设备（MRD），其允许测试不同的基质，并且试样可以在各种暴露时间之后独立地移除，用于检查生物膜或测试不同介质对原位生物膜生长的影响。不同类型岩石的薄片将被用作生物膜形成的天然基质。对于从深层地下流体中获得的假定嗜热菌和细菌的生物膜形成，指定了改进的可加热恒化器（系统B）。从这两个系统获得的生物膜将进行调查，其整体系统发育的结构，空间排列和细胞活性的生物膜相关的细菌使用荧光染料的总细胞计数测定和荧光原位杂交（FISH）。此外，对标志性脂质生物标志物的分析提供了对附着群落的丰度、分布和营养状况的定量测量。沿着微生物调查，将进行生物化学分析，特别是对流体（如脂质）中特定有机化合物的d13 C、d15 N、2H同位素组成的分析，这可能有助于阐明深层生物圈内微生物驱动的过程。