Analysis of molecular mechanisms of endoreduplication through chloroplast-dependent regulation.

重点研究了叶绿体控制内复制的分子机制。由于两个与叶绿体相关的基因被鉴定为显性突变体的对应基因，ilp2-D和ilp5-D表现出增加的多倍体。为了检验这些基因的生理功能，我们分析了这些基因突变体的丢失。然而，这些突变体在多倍体水平上没有表现出显著的表型。我们最近开发了一种独特的RNAi结构，用于敲除这些基因，并发表了一篇论文，描述了这种方法的几个优点。

We focused on the molecular mechanisms of endoreduplication controlled by chloroplast. Because two genes relating chloroplast were identified as the corresponding genes of dominant mutants, ilp2-D and ilp5-D that shows increased polyploidy. To examine physiological function of these genes, we analyzed loss of these genes mutants. However, these mutants did not showed significant phenotypes on polyploidy levels.We newly developed a unique construction of RNAi for the knock-down of these genes, and published a paper describing several advantage points of this method.