Obesity as a model of sex diversity in steroid metabolism (S03)

肥胖作为类固醇代谢性别多样性的模型（S03）

• 批准号: 536611999
• 金额: --
• 依托单位: Universitätskinderklinik
• 依托单位国家: 德国
• 项目类别: Collaborative Research Centres
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/536611999?language=en
• 关键词: Obesity; model; sex; diversity; steroid

The PIs of S03 will test the hypothesis that adipocytes from obese female subjects produce and release androgen-type steroids, and adipocytes from obese male subjects produce oestrogen-type steroids, resulting in a “pseudo-intersex steroid phenotype”. To this end, they will isolate preadipocytes and mature adipocytes from adipose tissue biopsies from lean female and male controls as well as obese female and male subjects. The cellular steroid hormone content as well as the secretome will be analysed using targeted LC-MS/MS and NMR spectroscopy (in collaboration with M06 and the hormone measurement service, as well as M05). The results of these ex vivo biopsy studies will then be tested in vivo using establisched human cohorts for obesity with cross-sectional, longitudinal and weight-loss intervention databases and biomaterials. Thereby the phenotyping will also include novel psycho-sozial phenotyping data.

S03的PI将检验以下假设：肥胖女性受试者的脂肪细胞产生并释放雄激素型类固醇，而肥胖男性受试者的脂肪细胞产生雌激素型类固醇，从而导致“伪间性类固醇表型”。为此，他们将从瘦女性和男性对照以及肥胖女性和男性受试者的脂肪组织活检中分离前脂肪细胞和成熟脂肪细胞。将使用靶向 LC-MS/MS 和 NMR 光谱（与 M06 和激素测量服务以及 M05 合作）分析细胞类固醇激素含量和分泌组。然后，这些离体活检研究的结果将使用已建立的肥胖人类队列、横截面、纵向和减肥干预数据库和生物材料进行体内测试。因此，表型分析还将包括新的心理社会表型数据。