Obesity as a model of sex diversity in steroid metabolism (S03)

S 03的PI将检验以下假设：肥胖女性受试者的脂肪细胞产生并释放雄激素型类固醇，肥胖男性受试者的脂肪细胞产生雌激素型类固醇，导致“假两性类固醇表型”。为此，他们将从瘦女性和男性对照以及肥胖女性和男性受试者的脂肪组织活检中分离前脂肪细胞和成熟脂肪细胞。将使用靶向LC-MS/MS和NMR光谱法（与M06和激素测量服务以及M05合作）分析细胞类固醇激素含量以及分泌组。这些离体活检研究的结果将在体内使用已建立的肥胖症人类队列进行测试，包括横截面，纵向和减肥干预数据库和生物材料。因此，表型分析还将包括新的心理-社会表型分析数据。

The PIs of S03 will test the hypothesis that adipocytes from obese female subjects produce and release androgen-type steroids, and adipocytes from obese male subjects produce oestrogen-type steroids, resulting in a “pseudo-intersex steroid phenotype”. To this end, they will isolate preadipocytes and mature adipocytes from adipose tissue biopsies from lean female and male controls as well as obese female and male subjects. The cellular steroid hormone content as well as the secretome will be analysed using targeted LC-MS/MS and NMR spectroscopy (in collaboration with M06 and the hormone measurement service, as well as M05). The results of these ex vivo biopsy studies will then be tested in vivo using establisched human cohorts for obesity with cross-sectional, longitudinal and weight-loss intervention databases and biomaterials. Thereby the phenotyping will also include novel psycho-sozial phenotyping data.