权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Effect of durotaxis-dependent cell movement in wound on development of keloid

伤口内杜罗轴依赖性细胞运动对瘢痕疙瘩发生的影响

基本信息

批准号：
19592068
负责人：
YOSHIMOTO Shinya
金额：
$ 2.83万
依托单位：
Chiba University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2007
资助国家：
日本
起止时间：
2007 至 2009
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19592068/
关键词：
創傷治癒学ケロイドデュロタキシスメカニカルストレス創傷治癒繊維我細胞テトラスパニン表皮角化細胞繊維芽細胞テトラスパニンウェッブスクラッチモデル皮膚由来線維芽細胞 MAPキナーゼ細胞外マトリックス

项目摘要

Keloids are skin fibrotic conditions that can be caused by minor insults to skin, such as acne or ear piercing, or by severe injuries such as burns. Keloids are characterized by an overabundant deposition of extra-cellular matrix such as collagen, and their histologic morphology has a characteristic manner. Keloid occurs more often on chest, shoulder or elbow-joint etc. which is easily affected by mechanical stretch. So we consider that the response to mechanical stress of fibroblast derived from Keloid may be different from that of normal skin fibroblast. We analyzed the sensitivity to mechanical stress using cell stretch apparatus (NC-500) which has silicon chambers on which the fibroblasts are cultured and scratched (We called wound repair scratch model). We found that wound repair is deffernted vertical and horizontary scratch. Furthermore knockdown of CD151 and EWI-F tetraspanin web moles induced detachment of cells from a culture apparatus.This effect is higher in keratinocytes than in keroid fibroblasts and normal fibroblasts. Therefore, we suggest that tetraspanin web moles affect skin wound healing.

瘢痕疙瘩是一种皮肤纤维性疾病，可由皮肤上的轻微侮辱引起，如粉刺或耳洞，或严重损伤，如烧伤。瘢痕疙瘩以胶原等细胞外基质过度沉积为特征，其组织学形态具有一定的特点。瘢痕疙瘩多发生在胸部、肩部或肘关节等部位，易受机械牵拉的影响。因此，我们认为瘢痕疙瘩成纤维细胞对机械应力的反应可能与正常皮肤成纤维细胞不同。我们用细胞拉伸仪(NC-500)分析了细胞对机械应力的敏感性，该设备上有硅室，成纤维细胞在硅室上培养和划痕(我们称之为创伤修复划痕模型)。我们发现创面修复有垂直划痕和水平划痕。此外，敲除CD151和EWI-F Tetraspanin网状葡萄胎会导致细胞从培养装置中分离，这种作用在角质形成细胞中比在角质形成纤维细胞和正常成纤维细胞中更高。因此，我们认为四环素网状葡萄胎会影响皮肤伤口的愈合。