Histological analysis of stress reaction of the stem/progenitor cell and those niche in periodontal ligament

牙周膜干/祖细胞及微环境应激反应的组织学分析

• 批准号: 19592138
• 负责人: SHIMADA Akemi
• 金额: $ 3万
• 依托单位: Tsurumi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19592138/
• 关键词: 歯根膜; 組織幹細胞; BrdU; label-retaining cell; 再植

It is thought that the tissue stem cells slowly proliferate in the normal condition, resulting that those retain DNA label for longer time than developed cells. We used BrdU to identify the label retaining cells (LRCs) as the tissue stem cells. In the mouse periodontal ligament, BrdU-LRCs distributed around blood vessels and near cementum. The BrdU-LRCs started to proliferate one day after replantation of the molar tooth, showing that the BrdU-LRCs possibly function to regenerate the periodontal tissue. It was also found that the collagen gel culture is useful to expand the BrdU-LRCs.

一般认为，组织干细胞在正常情况下增殖缓慢，导致其保留DNA标签的时间比发育细胞长。我们使用BrdU鉴定标记保留细胞（lrc）为组织干细胞。在小鼠牙周韧带中，brdu - lrc分布在血管周围和牙骨质附近。BrdU-LRCs在臼齿再植1天后开始增殖，表明BrdU-LRCs可能具有牙周组织再生的功能。同时发现胶原凝胶培养有利于brdu - lrc的扩增。