Histological analysis of annexin a5 function in the process ofperiodontal tissues regeneration.

牙周组织再生过程中膜联蛋白a5功能的组织学分析

• 批准号: 22592053
• 负责人: SHIMADA Akemi
• 金额: $ 2.66万
• 依托单位: Tsurumi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22592053/
• 关键词: 歯根膜; 組織幹細胞; アネキシン a5; アネキシンA5（Anxa5）; 腱; アポトーシス; アネキシンA5(Anxa5); β-galactosidase

Localization and function of Annexin a5 (Anxa5) expressing cells in the periodontal tissues were histologically analyzed. At 1 week after birth, Anxa5 was expressed at the area for tooth root formation. At 4 weeks after birth, Anxa5 was expressed in apex and cervical areas of tooth roots, cementblasts, periosteum, and perivascular areas. In the process of periodontal regeneration after tooth replantation at 6 weeks after birth, Anxa5 positive cells were markedly increased in the periodontal tissues at 3 days after replantation. At 8 weeks after birth, Anxa5 deficient mice showed less cement formation at the root apexes and wider periodontal ligament than those of wild mice. On the other hand, adult long bones of Anxa5 deficient mice showed larger bone formation at the insertionsites of tendon and ligament than those of wild mice. These results suggest that Anxa5 is involved in the process of development, remodeling and regeneration of hard tissues including surrounding soft tiss

对牙周组织中Annexin a5（Anxa5）表达细胞的定位和功能进行组织学分析。出生后1周，Anxa5在牙根形成区表达。出生后4周，Anxa5在牙根尖和颈部区域、牙骨质母细胞、骨膜和血管周围区域表达。在生后6周牙再植后牙周组织再生过程中，再植后3d牙周组织中Anxa 5阳性细胞明显增多。出生后8周，Anxa5缺陷小鼠的根尖骨水泥形成较少，牙周膜较野生小鼠宽。另一方面，Anxa 5缺陷小鼠的成年长骨在肌腱和韧带的附着点处显示出比野生小鼠更大的骨形成。这些结果提示Anxa5参与了包括周围软组织在内的硬组织的发育、重塑和再生过程

项目成果

Pre-incubation of whole teeth in collagen gel enrich progenitor cells of mouseperiodontal ligament.

在胶原凝胶中预孵育整颗牙齿可丰富小鼠牙周膜的祖细胞。

• 发表时间: 2011
• 作者: Shimada A;Shibata T;Komatsu K;Ideno H;Nifuji A.
• 通讯作者: Nifuji A.

Collagen Gel Culture Accelerates Proliferation of Mouse Periodontal Ligament Cells.

胶原凝胶培养加速小鼠牙周膜细胞的增殖。

• 发表时间: 2010
• 作者: Shimada A;Shibata T;Komatsu K;Nifuji A
• 通讯作者: Nifuji A

Expression of three histone 3 lysine 9 methyltransferases duringdevelopment of mouse molar

小鼠磨牙发育过程中三种组蛋白3赖氨酸9甲基转移酶的表达

• 发表时间: 2012
• 作者: Kamiunten T;Shimada A;Ideno H;Nakamura Y;Kimura H;Nakashima K;Nifuji A.
• 通讯作者: Nifuji A.

Annexin a5 Expression Regulates Periodontal Tissue and Cementum Formation

膜联蛋白 a5 表达调节牙周组织和牙骨质形成

• 发表时间: 2011
• 作者: Akemi Shimada;Koichiro Komatsu;Kazuhisa Nakashima;Norio Amizuka;Bent Brachvogel;Akira Nifuji
• 通讯作者: Akira Nifuji

Soluble factors may mediate signals from tendons to bone

可溶性因子可能介导从肌腱到骨骼的信号

• 发表时间: 2012
• 作者: Wada S;Shimada A;Ideno H;Nakamura Y;Nakashima K;Nifuji A.
• 通讯作者: Nifuji A.