Studies of genomics and proteomics for pathologic factors in temporomandibular joint

颞下颌关节病理因素的基因组学和蛋白质组学研究

• 批准号: 19592318
• 负责人: KONDOH Toshirou
• 金额: $ 2.83万
• 依托单位: Nihon University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19592318/
• 关键词: 顎関節内障; 顎関節滑膜細胞; GeneChip解析; Antibody Array; Chemokine; interluekin-1β; tumor necrosis factor-α; TNF-α; 滑膜炎モデル; IL-1β; IL-6; COX-2; PGE2; ラット顎関節OAモデル; microarray analysis

Synovitis that often accompanies intracapsular pathologic conditions is characterized by chronic inflammatory changes in the temporomandibular joint (TMJ). We have investigated of IL-1β or TNF-α-responsive genes in synoviocytes for putative genes associated with the synovitis using microarray analysis because IL-1b and TNF-a elevated in synovial fluids obtained from TMJ with pathologic conditions. Then, the protein levels of IL-1β or TNF-α-responsive factors have been investigated in synovitis models in vitro and in vivo.

滑膜炎通常伴随囊内病理状况，其特征是颞下颌关节 (TMJ) 的慢性炎症变化。我们使用微阵列分析研究了滑膜细胞中与滑膜炎相关的推定基因的 IL-1β 或 TNF-α 反应基因，因为从病理条件下的 TMJ 获得的滑液中 IL-1b 和 TNF-a 升高。然后，在体外和体内滑膜炎模型中研究了 IL-1β 或 TNF-α 反应因子的蛋白水平。