Neuronal Modulation after long term forepaw immobilization in rats.

大鼠长期前爪固定后的神经元调节。

• 批准号: 19603003
• 负责人: USIDA Takahiro
• 金额: $ 2.25万
• 依托单位: Aichi Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19603003/
• 关键词: 関節拘縮; 不動化; 運動負荷; 脊髄後角; 拘縮; 廃用; Substance P; CGRP; 脊髄感作

Long-term immobilization by casting can occasionally cause pathologic pain states in the immobilized side. The underlying neurophysiological mechanisms of immobilization-related pain are not well understood. For this reason, we specifically examined changes of calcitonin gene-related peptide (CGRP) expression in the dorsal root ganglion (DRG), spinal dorsal horn and posterior nuclei (cuneate nuclei) ina long-term immobilization model following casting for 5 weeks. A plastic cast was wrapped around the right limb from the forearm to the forepaw to keep wrist joint at 90 degree of flexion. In this model, CGRP in immobilized (ipsilateral) side was distributed in larger DRG neurons compared with contralateral side, even though the number of CGRP-immunoreactive (CGRP-IR) neurons did not differ. Spinal laminae III-V, not laminae I-II in ipsilateral side showed significantly high CGRP expression relative to contralateral side. In addition expression of GFSP and CD11b were increased after long term immobilization. These results suggest that neuronal plastic changes were occurred after long term casting addition to joint contracture. Additional casting to sound forepaw was useful method to improve disuse behavior after the cast treatment. Thus adequate musculoskeletal rehabilitation was essential for the treatment.

长期的石膏固定偶尔会引起固定侧的病理性疼痛状态。制动相关疼痛的潜在神经生理学机制还不清楚。为此，我们专门研究了降钙素基因相关肽（CGRP）表达的变化，背根神经节（DRG），脊髓背角和后核（楔状核）在长期制动模型铸造后5周。将塑料铸件从前臂到前爪缠绕在右肢周围，以保持腕关节处于90度屈曲。在这个模型中，CGRP在固定（同侧）侧分布在较大的DRG神经元与对侧相比，即使CGRP免疫反应（CGRP-IR）神经元的数量没有差异。与对侧相比，同侧脊髓III-V层CGRP表达显著增高，而I-II层无明显变化。此外，长期制动后GFSP和CD 11b表达增加。这些结果表明，关节挛缩后，长期铸造除了神经元的可塑性变化。对健全前爪进行石膏固定是改善石膏固定后废用行为的有效方法。因此，充分的肌肉骨骼康复是必不可少的治疗。