Gene expression noise during cell differentiation and its regulation

细胞分化过程中的基因表达噪声及其调控

• 批准号: 20200006
• 负责人: YAMAMOTO Takashi
• 金额: $ 18.64万
• 依托单位: Hiroshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research on Innovative Areas (Research a proposed research project)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20200006/
• 关键词: ゆらぎ; 細胞分化; 発生・分化; 遺伝子; ゲノム; 発現制御

In order to analyze the gene expression level during cell differentiation quantitatively, we established the reporter-knockin system into specific site of genome using engineered zinc-finger nucleases (ZFNs). Using this technology, we inserted reporter genes into target sites within the sea urchin Ets (HpEts) gene, which is expressed in presumptive primary mesenchyme cells (PMCs) and PMCs, and monitored the expression at one cell level during development, and found that gene expression noise increased during PMC differentiation in sea urchin development.

为了定量分析细胞分化过程中的基因表达水平，我们利用工程化锌指核酸酶（ZFN）建立了基因组特定位点的报告基因敲入系统。使用这种技术，我们插入报告基因的海胆Ets（HpEts）基因，这是在假定的初级间充质细胞（PMC）和PMC中表达的目标位点，并监测在一个细胞水平上的表达在发展过程中，发现基因表达噪音增加PMC分化过程中海胆发展。

项目成果

Implication of HpEts in gene regulatory networks responsible for specification of sea urchin skeletogenic primary mesenchyme cells.

HpEts 在负责海胆成骨原代间充质细胞规范的基因调控网络中的意义。

• 发表时间: 2010
• 期刊: Zoological Science 27(8)
• 作者: Yajima M;Umeda R;Fuchikami T;Kataoka M;Sakamoto N;Yamamoto T;Akasaka K.
• 通讯作者: Akasaka K.

Identification and characterization of nucleoplasmin 3 as a histone-binding protein in embryonic stem cells

• DOI: 10.1111/j.1440-169x.2008.01034.x
• 发表时间: 2008-06-01
• 期刊: DEVELOPMENT GROWTH & DIFFERENTIATION
• 影响因子: 2.5
• 作者: Motoi, Natsuki;Suzuki, Ken-ichi;Yoshizato, Katsutoshi
• 通讯作者: Yoshizato, Katsutoshi

Mammalian arlysulfatase A functions as a novel component of the extracellular matrix.

哺乳动物芳基硫酸酯酶 A 作为细胞外基质的新成分发挥作用。

• 发表时间: 2010
• 期刊: Connective Tissue Research
• 影响因子: 2.9
• 作者: Nishikawa M;Shibata T.;Ochiai H.;Fujita K.;Fujita K.
• 通讯作者: Fujita K.

Targeted manipulation of genes with zinc finger nucleases in the cricket, Gryllus bimaculatus

用锌指核酸酶对蟋蟀 Gryllus bimaculatus 中的基因进行靶向操作

• 发表时间: 2010
• 作者: Nishikawa M;Takagi H;Shibata T;Iwane AH;Yanagida T.;Ochiai H.;Motoi N.;Shibata T.;Ishihara S.;Nishikawa M.;Watanabe Takahito
• 通讯作者: Watanabe Takahito

Relationship between the noise in adaptation reaction and the chemotactic performance in bacterium

细菌适应反应噪声与趋化性能的关系

• 发表时间: 2009
• 作者: Nishikawa M;Takagi H;Shibata T;Iwane AH;Yanagida T.;Ochiai H.;Motoi N.;Shibata T.;Ishihara S.;Nishikawa M.;Watanabe Takahito;Watanabe T.;Takebayashi-Suzuki Kimiko;吉田隆也;藤田和将;西川正俊;Nishikawa Masatoshi;藤田和将;山本卓;西川正俊;Nishikawa Masatoshi
• 通讯作者: Nishikawa Masatoshi