Microelectrode Techniques for Cellular Engineering

我们已经成功地开发了一种在纤维蛋白凝胶片上培养肌管的技术。制备的肌管在水凝胶上的培养在电刺激下稳定收缩1周以上。我们还成功地在胶原和琼脂糖等水凝胶上制备了PEDOT微电极，其表面电阻率为50Ω/-。这两个水凝胶片的结合，肌管和PEDOT被微图案化，提供了一个完全收缩的生物芯片，将成为肌肉靶向药物开发的强大工具。

We have succeeded in developing a technique to culture myotubes on a fibrin gel sheet. The prepared myotube culture on the hydrogel stably contract on electrical stimulation for more than 1 week. We also succeeded in preparing PEDOT microelectrodes on hydrogels like collagen and agarose, that shows surface resistivity of 50 Ω/□. The combination of these two hydrogel sheets, on which myotubes and PEDOT are micropatterned, provides a totally contractile biochip that will be a powerful tool for the muscle-targeted drug development.