Basic research of high-throughput-technology which adds a small amount of liquid reagents using impact force generated with femtosecond laser

我们开发了使用飞秒激光(飞秒激光)刺激和操纵活细胞的基本技术。1)通过施加飞秒激光诱导的撞击，将拖拽物输送到培养基中的有限区域，以用拖拽物轰炸靶细胞。2)撞击可以将目标细胞从培养板中释放出来，并将其输送到所需位置。3)将飞秒激光聚焦到涂有疏水性聚合物的培养平台上，培养平台的表面特性由疏水性变为亲细胞性。综上所述，这种水溶液中的飞秒激光过程实现了有限的细胞刺激和多细胞排列。

We developed fundamental technologies by using a femtosecond laser(fs laser) on living-cell stimulation and manipulation. 1) Drags were delivered to limited area in culture medium by applying an fs laser-induced impact for bombing targeted cells with the drags. 2) The impact could release a targeted cell from a culture plate and delivered to a desired position. 3) Surface characteristic of the culture platform was changed from cytophobic to cytophilic when the fs laser was focused to the platform coated with cytophobic polymer. In conclusion, this fs-laser process in aqueous solution achieved the limited cell stimulation and the multiple cell arrangement.