Basic research of high-throughput-technology which adds a small amount of liquid reagents using impact force generated with femtosecond laser

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We developed fundamental technologies by using a femtosecond laser(fs laser) on living-cell stimulation and manipulation. 1) Drags were delivered to limited area in culture medium by applying an fs laser-induced impact for bombing targeted cells with the drags. 2) The impact could release a targeted cell from a culture plate and delivered to a desired position. 3) Surface characteristic of the culture platform was changed from cytophobic to cytophilic when the fs laser was focused to the platform coated with cytophobic polymer. In conclusion, this fs-laser process in aqueous solution achieved the limited cell stimulation and the multiple cell arrangement.