Control of Orientation and Shape of Adult Stem Cell on Base Surface by Negative-Ion Pattern Implantation and Induction of The Differentiation

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Mesenchymal stem cells (MSC) derived from bone-mallow of rat showed their self-aligned adhesion on polymer surfaces along the modified line-region in which carbon negative-ions were implanted through line-pattern mask for improving hydrophilicity. Besides, the adhered cells were differentiated into nerve cells and osteoblast cells with holding their adhesion pattern. By narrowing the width of the line pattern, the orientation of MSC cell body and nuclei were controlled to along with the long direction of the line pattern of the ion implantation. Cell sheet and string were formed by using C and O negative ion implantation into poly-lactic acid.