Control of Orientation and Shape of Adult Stem Cell on Base Surface by Negative-Ion Pattern Implantation and Induction of The Differentiation

负离子模式植入控制成体干细胞基面方向和形状及诱导分化

• 批准号: 20360018
• 负责人: TSUJI Hiroshi
• 金额: $ 11.9万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20360018/
• 关键词: 負イオン注入; 表面改質; 細胞親和性; 間葉系幹細胞; 神経回路網; 細胞チップ; 負イオン注入処理; パターン接着; 親疎水性制御; 自発的パターン配列接着; 分化誘導; 骨芽細胞; パターン表面改質; 生体適合性制御; 石英ガラス; 人為的神経回路網

Mesenchymal stem cells (MSC) derived from bone-mallow of rat showed their self-aligned adhesion on polymer surfaces along the modified line-region in which carbon negative-ions were implanted through line-pattern mask for improving hydrophilicity. Besides, the adhered cells were differentiated into nerve cells and osteoblast cells with holding their adhesion pattern. By narrowing the width of the line pattern, the orientation of MSC cell body and nuclei were controlled to along with the long direction of the line pattern of the ion implantation. Cell sheet and string were formed by using C and O negative ion implantation into poly-lactic acid.

源自大鼠骨髓的间充质干细胞（MSC）显示出沿着修饰的线区域在聚合物表面上的自对准粘附，其中通过线图案掩模植入碳负离子以改善亲水性。此外，粘附细胞分化为神经细胞和成骨细胞，并保持其粘附模式。通过缩小线图案的宽度，将MSC细胞体和细胞核的取向控制为沿着离子注入的线图案的长方向。通过将C和O负离子注入聚乳酸中形成细胞片和​​串。

项目成果

Mesenchymal Stem Cell Patterning and Its Differentiation into Neuron on Silicone Rubber Modified by Carbon Negative-IoImplantation

碳负离子注入改性硅橡胶上间充质干细胞图案化及其向神经元分化

• 发表时间: 2009
• 作者: P.Sommani;H.Tsuji;H.Sato;T.Yamada;H.Yamada;Y.Gotoh;J.Ishikawa
• 通讯作者: J.Ishikawa

Negative-Ion Beam Modified Silicone Rubber for Patterning Adhesions of Mesenchymal Stem Cell and Its Differentiated Neurons

负离子束改性硅橡胶用于间充质干细胞及其分化神经元的图案化粘附

• 发表时间: 2008
• 作者: P.Sommani;H.Tsuji;H.Sato;T.Yamada;H.Kojima;Y.Gotoh;J.Ishikawa
• 通讯作者: J.Ishikawa

Osteoblast Patterning on Silicone Rubber by using Mesenchymal Stem Cells and Carbon Negative-Ion

使用间充质干细胞和碳负离子在硅橡胶上形成成骨细胞图案

• 发表时间: 2010
• 作者: P.Sommani;H.Tsuji;H.Sato;Y.Gotoh;J.Ishikawa;G.H.Takaoka
• 通讯作者: G.H.Takaoka

Negative-Ion Beam Modification of Silicone Rubber for Patterning Osteoblast from Differentiation of Mesenchymal Stem Cells

负离子束改性硅橡胶用于间充质干细胞分化成骨细胞的图案化

• 发表时间: 2010
• 作者: Plyanuch Sommanil;Hiroshi Tsuji;Hiroko Sato;Yasuhito Gotoh;Gikan Takaoka
• 通讯作者: Gikan Takaoka

Cell Adhesion Pattering on Polystyrene by Using Avidin Coating and Carbon Negative-Ion Implantation

使用抗生物素蛋白涂层和碳负离子注入在聚苯乙烯上形成细胞粘附图案

• 发表时间: 2009
• 作者: P.Sommani;H.Tsuji;H.Sato;Y.Gotoh;J.Ishikawa;G.H.Takaoka
• 通讯作者: G.H.Takaoka